Impact of intravenous/intranasal polyinosinic-polycytidylic acid administration on the mediastinal fat-associated lymphoid clusters and lung tissue in healthy mice.

Autor: Elewa YHA; Faculty of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan; Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt. Electronic address: y-elewa@vetmed.hokudai.ac.jp., Khalifa AM; Laboratory of Innovative Nanomedicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan., Zahran MH; Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt.
Jazyk: angličtina
Zdroj: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft [Ann Anat] 2023 Oct; Vol. 250, pp. 152158. Date of Electronic Publication: 2023 Sep 02.
DOI: 10.1016/j.aanat.2023.152158
Abstrakt: Background: Polyinosinic-polycytidylic acid (pIC) is a synthetic analog of double-stranded RNA. It is used as a synthetic adjuvant to induce an adaptive immune response. However, the effect of pIC on the development of mediastinal fat-associated lymphoid clusters (MFALCs) that regulate intrathoracic hemostasis has remained unidentified.
Methods: We investigated the impact of intranasal (i.n.) administration (pIC i.n. group) and intravenous (i.v.) administration (pIC i.v. group) of pIC on both MFALCs and lung tissue.
Results: Compared with the control phosphate-buffered saline (PBS) groups, both pIC-administered groups displayed a significant increase in the MFALC size (particularly in the pIC i.n. group), area of MFALC high endothelial venules (HEVs), area of lymphatic vessels (LVs), number of proliferating cells (particularly in the pIC i.v. group), and number of immune cells (B220 + B-lymphocytes, CD3 + T-lymphocytes, Iba1 + macrophages, and Gr-1 + granulocytes) in both MFALCs and lung tissues. In addition, a positive correlation was detected between MFALC size and proliferating cells, immune cell population, LVs, and HEVs within MFALCs in both groups. Except for the proliferating cell and B-lymphocyte populations in the i.n. administered group and granulocyte populations in both i.n. and i.v. administered routes, such correlations were significant.
Conclusion: In all, our data indicate that local or systemic administration of pIC induces the development of MFALCs and can be used as an immunostimulant therapeutic strategy.
Competing Interests: Declaration of Competing Interest The authors declare no competing financial interests.
(Copyright © 2023 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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