| Autor: |
Rochard V; Laboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l'Eau, F-86073 Poitiers, France., Cochard T; Institut National de Recherche pour l'Agriculture-INRAE, Université de Tours, ISP, F-37390 Nouzilly, France., Crapart S; Laboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l'Eau, F-86073 Poitiers, France., Delafont V; Laboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l'Eau, F-86073 Poitiers, France., Moyen JL; Laboratoire Départemental d'Analyse et de Recherche de la Dordogne, F-24660 Coulounieix-Chamiers, France., Héchard Y; Laboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l'Eau, F-86073 Poitiers, France., Biet F; Institut National de Recherche pour l'Agriculture-INRAE, Université de Tours, ISP, F-37390 Nouzilly, France. |
| Abstrakt: |
One of the obstacles to eradicating paratuberculosis or Johne's Disease (JD) seems to be the persistence of Mycobacterium avium subsp. paratuberculosis ( Map ) in the environment due to its ability to survive alone or vectorized. It has been shown that Map is widely distributed in soils and water. Previously, we isolated amoebae associated with Map strains in the environment of bovines from an infected herd. This work aims to verify our working hypothesis, which suggests that amoebae may play a role in the transmission of JD. In this study, we sampled water in the vicinity of herds infected with Map or Mycobacterium bovis ( M. bovis ) and searched for amoebae and mycobacteria. Live amoebae were recovered from all samples. Among these amoebae, four isolates associated with the presence of mycobacteria were identified and characterized. Map and other mycobacterial species were detected by qPCR and, in some cases, by culture. This study suggests that amoebae and Map may be found in the same environment and might represent a risk of exposure of animals to pathogenic mycobacteria. These data open up new perspectives on the control measures to be put in place to prevent contamination by Map . |
