Improving diagnosis of non-malarial fevers in Senegal: Borrelia and the contribution of tick-borne bacteria.

Autor:	Levine ZC; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Harvard Graduate Program in Biological and Biomedical Science, Boston, MA, USA.; Harvard/MIT MD-PhD Program, Boston, MA, 02115, USA., Sene A; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Mkandawire W; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; University of Massachusetts Medical School, Worcester, MA, USA., Deme AB; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Ndiaye T; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Sy M; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Gaye A; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Diedhiou Y; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Mbaye AM; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Ndiaye I; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Gomis J; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Ndiop M; Programme National de Lutte contre le Paludisme (PNLP), Ministère de la Santé, Dakar Fann, Senegal., Sene D; Programme National de Lutte contre le Paludisme (PNLP), Ministère de la Santé, Dakar Fann, Senegal., Paye MF; Broad Institute of Harvard and MIT, Cambridge, MA, USA., MacInnis B; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA, USA., Schaffner SF; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA., Park DJ; Broad Institute of Harvard and MIT, Cambridge, MA, USA., Badiane AS; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Colubri A; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; University of Massachusetts Medical School, Worcester, MA, USA., Ndiaye M; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Sy N; Service de Lutte Anti Parasitaire, Thies, Senegal., Sabeti PC; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Harvard University, Boston, MA, USA.; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA., Ndiaye D; Centre International de recherche, de formation en Génomique Appliquée et de Surveillance Sanitaire (CIGASS), Dakar, Senegal., Siddle KJ; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Molecular Microbiology and Immunology, Brown University, Providence, RI, USA.
Jazyk:	angličtina
Zdroj:	MedRxiv : the preprint server for health sciences [medRxiv] 2023 Aug 25. Date of Electronic Publication: 2023 Aug 25.
DOI:	10.1101/2023.08.24.23294564
Abstrakt:	The worldwide decline in malaria incidence is revealing the extensive burden of non-malarial febrile illness (NMFI), which remains poorly understood and difficult to diagnose. To characterize NMFI in Senegal, we collected venous blood and clinical metadata from febrile patients and healthy controls in a low malaria burden area. Using 16S and unbiased sequencing, we detected viral, bacterial, or eukaryotic pathogens in 29% of NMFI cases. Bacteria were the most common, with relapsing fever Borrelia and spotted fever Rickettsia found in 15% and 3.7% of cases, respectively. Four viral pathogens were found in a total of 7 febrile cases (3.5%). Sequencing also detected undiagnosed Plasmodium , including one putative P. ovale infection. We developed a logistic regression model to distinguish Borrelia from NMFIs with similar presentation based on symptoms and vital signs. These results highlight the challenge and importance of improved diagnostics, especially for Borrelia , to support diagnosis and surveillance. Competing Interests: Competing Interests P.C.S. is a co-founder of, shareholder in, and consultant to Sherlock Biosciences, Inc. and Delve Bio, as well as a Board member of and shareholder in Danaher Corporation.
Databáze:	MEDLINE
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