Ultra-high density electrodes improve detection, yield, and cell type identification in neuronal recordings.

Autor:	Ye Z; Department of Biological Structure, University of Washington, Seattle, WA, USA., Shelton AM; MindScope Program, Allen Institute, Seattle, WA, USA.; Allen Institute for Neural Dynamics, Seattle, WA, USA., Shaker JR; Department of Biological Structure, University of Washington, Seattle, WA, USA., Boussard J; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Colonell J; Janelia Research Campus, Ashburn, VA, USA., Birman D; Department of Biological Structure, University of Washington, Seattle, WA, USA., Manavi S; MindScope Program, Allen Institute, Seattle, WA, USA., Chen S; Janelia Research Campus, Ashburn, VA, USA., Windolf C; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Hurwitz C; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Namima T; Department of Biological Structure, University of Washington, Seattle, WA, USA.; Washington National Primate Research Center, Seattle, WA, USA., Pedraja F; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Weiss S; Max Planck Institute for Brain Research, Frankfurt, Germany., Raducanu B; IMEC, Leuven, Belgium., Ness TV; Norwegian University of Life Sciences, Ås, Norway., Jia X; Center for Life Sciences & IDG/McGovern Institute for Brain Research, Tsinghua University, China., Mastroberardino G; UCL Institute of Ophthalmology, University College London, London, UK.; Wolfson Institute for Biomedical Research, University College London, London, UK., Rossi LF; Center for Neuroscience and Cognitive Systems, Istituto Italiano di Tecnologia, Rovereto, Italy., Carandini M; UCL Institute of Ophthalmology, University College London, London, UK., Häusser M; Wolfson Institute for Biomedical Research, University College London, London, UK., Einevoll GT; Norwegian University of Life Sciences, Ås, Norway.; University of Oslo, Oslo, Norway., Laurent G; Max Planck Institute for Brain Research, Frankfurt, Germany., Sawtell NB; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Bair W; Department of Biological Structure, University of Washington, Seattle, WA, USA.; Washington National Primate Research Center, Seattle, WA, USA., Pasupathy A; Department of Biological Structure, University of Washington, Seattle, WA, USA.; Washington National Primate Research Center, Seattle, WA, USA., Lopez CM; IMEC, Leuven, Belgium., Dutta B; IMEC, Leuven, Belgium., Paninski L; Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA., Siegle JH; Allen Institute for Neural Dynamics, Seattle, WA, USA., Koch C; MindScope Program, Allen Institute, Seattle, WA, USA., Olsen SR; MindScope Program, Allen Institute, Seattle, WA, USA.; Allen Institute for Neural Dynamics, Seattle, WA, USA., Harris TD; Janelia Research Campus, Ashburn, VA, USA.; Biomedical Engineering Department, Johns Hopkins University, Baltimore, MD, USA., Steinmetz NA; Department of Biological Structure, University of Washington, Seattle, WA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Apr 10. Date of Electronic Publication: 2024 Apr 10.
DOI:	10.1101/2023.08.23.554527
Abstrakt:	To understand the neural basis of behavior, it is essential to sensitively and accurately measure neural activity at single neuron and single spike resolution. Extracellular electrophysiology delivers this, but it has biases in the neurons it detects and it imperfectly resolves their action potentials. To minimize these limitations, we developed a silicon probe with much smaller and denser recording sites than previous designs, called Neuropixels Ultra ( NP Ultra ). This device samples neuronal activity at ultra-high spatial density (~10 times higher than previous probes) with low noise levels, while trading off recording span. NP Ultra is effectively an implantable voltage-sensing camera that captures a planar image of a neuron's electrical field. We use a spike sorting algorithm optimized for these probes to demonstrate that the yield of visually-responsive neurons in recordings from mouse visual cortex improves up to ~3-fold. We show that NP Ultra can record from small neuronal structures including axons and dendrites. Recordings across multiple brain regions and four species revealed a subset of extracellular action potentials with unexpectedly small spatial spread and axon-like features. We share a large-scale dataset of these brain-wide recordings in mice as a resource for studies of neuronal biophysics. Finally, using ground-truth identification of three major inhibitory cortical cell types, we found that these cell types were discriminable with approximately 75% success, a significant improvement over lower-resolution recordings. NP Ultra improves spike sorting performance, detection of subcellular compartments, and cell type classification to enable more powerful dissection of neural circuit activity during behavior. Competing Interests: Declaration of interests CK holds an executive position, and has a financial interest, in Intrinsic Powers, Inc., a company whose purpose is to develop a device that can be used in the clinic to assess the presence and absence of consciousness in patients. This does not pose any conflict of interest with regard to the work undertaken for this publication. BR, CML, and BD are employees of IMEC vzw, a nonprofit research institute that manufactures, sells, and distributes the Neuropixels probes, at cost, to the research community. IMEC vzw holds patents US10811542B2, US10044325B2, and US9384990B2 related to the Neuropixels 1.0 technology that is built upon in this work. All other authors have no competing interests.
Databáze:	MEDLINE
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