Candidate pathogenicity factor/effector proteins of ' Candidatus Phytoplasma solani' modulate plant carbohydrate metabolism, accelerate the ascorbate-glutathione cycle, and induce autophagosomes.

Autor:	Dermastia M; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia., Tomaž Š; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.; Jožef Stefan International Postgraduate School, Ljubljana, Slovenia., Strah R; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.; Jožef Stefan International Postgraduate School, Ljubljana, Slovenia., Lukan T; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia., Coll A; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia., Dušak B; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.; Department of Plant and Environmental Sciences, University of Copenhagen, Taastrup, Denmark., Anžič B; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia., Čepin T; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia., Wienkoop S; Department of Functional and Evolutionary Ecology, Faculty of Life Sciences, University of Vienna, Vienna, Austria., Kladnik A; Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia., Zagorščak M; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia., Riedle-Bauer M; Federal College and Research Institute for Viticulture and Pomology Klosterneuburg, Klosterneuburg, Austria., Schönhuber C; Bioresources Unit, Health & Environment Department, Austrian Institute of Technology, Tulln, Austria., Weckwerth W; Department of Functional and Evolutionary Ecology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.; Vienna Metabolomics Center (VIME), University of Vienna, Vienna, Austria., Gruden K; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia., Roitsch T; Department of Plant and Environmental Sciences, University of Copenhagen, Taastrup, Denmark., Pompe Novak M; Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.; Faculty of Viticulture and Enology, University of Nova Gorica, Vipava, Slovenia., Brader G; Bioresources Unit, Health & Environment Department, Austrian Institute of Technology, Tulln, Austria.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Aug 18; Vol. 14, pp. 1232367. Date of Electronic Publication: 2023 Aug 18 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1232367
Abstrakt:	The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ' Candidiatus Phytoplasma solani' are unknown. Six putative pathogenicity factors/effectors from six different strains of ' Ca . P. solani' were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium -mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate-glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate-glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Dermastia, Tomaž, Strah, Lukan, Coll, Dušak, Anžič, Čepin, Wienkoop, Kladnik, Zagorščak, Riedle-Bauer, Schönhuber, Weckwerth, Gruden, Roitsch, Pompe Novak and Brader.)
Databáze:	MEDLINE
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