Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes.

Autor: Lukšíková K; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.; Department of Genetics and Microbiology, Faculty of Science, Charles University, Prague, Czech Republic., Pavlica T; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.; Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic., Altmanová M; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.; Department of Ecology, Faculty of Science, Charles University, Prague, Czech Republic., Štundlová J; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.; University of South Bohemia, Faculty of Science, České Budějovice, Czech Republic., Pelikánová Š; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic., Simanovsky SA; Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia., Krysanov EY; Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia., Jankásek M; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.; Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic., Hiřman M; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.; Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic., Reichard M; Institute of Vertebrate Biology, Czech Academy of Sciences, Czech Republic.; Department of Ecology and Vertebrate Zoology, University of Łódź, Łódź, Poland.; Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic., Ráb P; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic., Sember A; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, Czech Republic.
Jazyk: angličtina
Zdroj: Journal of fish biology [J Fish Biol] 2023 Dec; Vol. 103 (6), pp. 1501-1514. Date of Electronic Publication: 2023 Sep 19.
DOI: 10.1111/jfb.15550
Abstrakt: Using African annual killifishes of the genus Nothobranchius from temporary savannah pools with rapid karyotype and sex chromosome evolution, we analysed the chromosomal distribution of telomeric (TTAGGG) n repeat and Nfu-SatC satellite DNA (satDNA; isolated from Nothobranchius furzeri) in 15 species across the Nothobranchius killifish phylogeny, and with Fundulosoma thierryi as an out-group. Our fluorescence in situ hybridization experiments revealed that all analysed taxa share the presence of Nfu-SatC repeat but with diverse organization and distribution on chromosomes. Nfu-SatC landscape was similar in conspecific populations of Nothobranchius guentheri and Nothobranchius melanospilus but slightly-to-moderately differed between populations of Nothobranchius pienaari, and between closely related Nothobranchius kuhntae and Nothobranchius orthonotus. Inter-individual variability in Nfu-SatC patterns was found in N. orthonotus and Nothobranchius krysanovi. We revealed mostly no sex-linked patterns of studied repetitive DNA distribution. Only in Nothobranchius brieni, possessing multiple sex chromosomes, Nfu-SatC repeat occupied a substantial portion of the neo-Y chromosome, similarly as formerly found in the XY sex chromosome system of turquoise killifish N. furzeri and its sister species Nothobranchius kadleci-representatives not closely related to N. brieni. All studied species further shared patterns of expected telomeric repeats at the ends of all chromosomes and no additional interstitial telomeric sites. In summary, we revealed (i) the presence of conserved satDNA class in Nothobranchius clades (a rare pattern among ray-finned fishes); (ii) independent trajectories of Nothobranchius sex chromosome differentiation, with recurrent and convergent accumulation of Nfu-SatC on the Y chromosome in some species; and (iii) genus-wide shared tendency to loss of telomeric repeats during interchromosomal rearrangements. Collectively, our findings advance our understanding of genome structure, mechanisms of karyotype reshuffling, and sex chromosome differentiation in Nothobranchius killifishes from the genus-wide perspective.
(© 2023 Fisheries Society of the British Isles.)
Databáze: MEDLINE
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