Development of recombinant major capsid protein Vaccine and assessment of its efficacy against SRDV in similar damselfish (Pomacentrus similis).

Autor: Panchavarnam S; Department of Fish Pathology and Health Management, Fisheries College and Research Institute, Thoothukudi, 628 008, Tamil Nadu, India. Electronic address: sanknag@gmail.com., Kollanoor RJ; Department of Fish Pathology and Health Management, Fisheries College and Research Institute, Thoothukudi, 628 008, Tamil Nadu, India., Mulloorpeedikayil RG; Department of Fish Pathology and Health Management, Fisheries College and Research Institute, Thoothukudi, 628 008, Tamil Nadu, India., Mohaideenpitchai MM; Department of Fish Pathology and Health Management, Fisheries College and Research Institute, Thoothukudi, 628 008, Tamil Nadu, India., Palraj MK; Department of Fish Pathology and Health Management, Fisheries College and Research Institute, Thoothukudi, 628 008, Tamil Nadu, India., Muthumariyapan S; Department of Fish Pathology and Health Management, Fisheries College and Research Institute, Thoothukudi, 628 008, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2023 Oct; Vol. 141, pp. 109035. Date of Electronic Publication: 2023 Sep 01.
DOI: 10.1016/j.fsi.2023.109035
Abstrakt: Viral vaccines against emerging viral diseases are crucial for encouraging successful aquaculture production. In this research, an experimental recombinant major capsid protein vaccine of similar damselfish virus was prepared and examined for its efficacy in marine ornamental fish, similar damselfish (Pomacentrus similis). The MCP gene of the SRDV was amplified from the viral DNA by a specific primer set viz bamHI and XhoI- restriction sites and confirmed by agarose gel electrophoresis with a target size of 1416 bp. The gel-purified PCR product was double-digested with the said enzymes and incorporated into the pTriEx1.1 vector, which was subsequently transformed to E. coli DH5α. The plasmids of the two clones pTriEx-MCP-1416-1 and pTriEx-MCP-1416-3 were transformed to E. coli BL21 (DE-3) pLacI. A crude protein compound derived from a colony of E. coli BL21 (DE-3) with expressed MCP inserts was used to evaluate efficacy in similar damselfish by intra-peritoneal injection. After the challenge with SRDV, damselfish vaccinated with recombinant protein showed a lower protection level, while the fish vaccinated with recombinant protein supplemented Quil-A® adjuvant showed an RPS of 26%. According to RPS values recorded from the vaccinated and non-vaccinated damselfish group, the recombinant protein vaccine conferred only marginal protection against the SRDV challenge.
Competing Interests: Declaration of competing interest The authors have no conflict of interest to declare.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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