Ameliorating effect of Citrus trifoliata L. fruits extract on motor incoordination, neurodegeneration and oxidative stress in Parkinson's disease model.

Autor: Abdel-Kawy MA; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Aboulhoda BE; Anatomy and Embryology Department, Faculty of Medicine, Cairo University, Cairo, Egypt., Michel CG; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Sedeek MS; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Kirollos FN; Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Masoud MA; Pharmacology Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt.
Jazyk: angličtina
Zdroj: Nutritional neuroscience [Nutr Neurosci] 2024 Jul; Vol. 27 (7), pp. 770-782. Date of Electronic Publication: 2023 Sep 02.
DOI: 10.1080/1028415X.2023.2253026
Abstrakt: Background: Citrus trifoliate fruit (also known as Trifoliate orange) is one of the commercially-cultivated Citrus genus of plants belonging to the Rutaceae family. It has been traditionally-utilized in treatment of neurodegenerative disorders. However, the scientific evidence verifying this utilization needs further elucidation.
Aim of the Study: Characterization of the bioactive constituents of C. trifoliata L. fruits extract and evaluating its effect on Parkinson's disease (PD) model.
Material and Methods: Rats were classified into 5 groups; control, PD, PD-treated by L-dopa/Carpidopa and PD-treated by oral Citrus trifoliata L. fruits extract (50 and 100 mg/kg). Deterioration in brain functions was evaluated through an in vivo open field, grid and catalepsy tests. The study also assessed the striatal neurotransmitters, oxidative stress markers and histopathological changes.
Results: Citrus trifoliata L. fruit extract has revealed motor improvement comparable to L-dopa and carbidopa. It has also effectively-improved oxidative stress via reduction of striatal malondialdehyde & nitric oxide along with replenishment of the striatal glutathione and superoxide dismutase . The extract caused significant reduction of the striatal myeloperoxidase activity and restoration of dopamine, γ-amino butyric acid (GABA), and acetylcholinesterase. This effect was further confirmed by amelioration of neuronal apoptosis, microgliosis and peri-neuronal vacuolation. Metabolite profiling revealed 40 constituents, with flavonoids representing the main identified class.
Conclusion: The neuro-protective effect of Citrus trifoliata extract was achieved through the antioxidant and anti-inflammatory activities of its flavonoids, particularly hesperidin and naringin. This neuro-protective effect was evident at the behavioral, histological and neurotransmitter levels.
Databáze: MEDLINE
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