MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues.

Autor: Cheng Z; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Stefani C; Benaroya Research Institute at Virginia Mason, 1201 9th Ave, Seattle, WA, 98101, USA., Skillman T; Immersive Science LLC, 6835 113TH PL SE, Newcastle, WA, 98056, USA., Klimas A; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Lee A; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., DiBernardo EF; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Brown KM; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Milman T; Wills Eye Hospital and Jefferson University Hospital, Philadelphia, PA, 19107, USA., Wang Y; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Gallagher BR; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Lagree K; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Jena BP; Viron Molecular Medicine Institute, 201 Washington Street, Boston, MA, 02201, USA.; Department of Physiology, Wayne State University, 42 W Warren Ave, Detroit, MI, 48202, USA.; NanoBioScience Institute, Wayne State University, 42 W Warren Ave, Detroit, MI, 48202, USA.; Center for Molecular Medicine & Genetics, School of Medicine, Wayne State University, 42 W Warren Ave, Detroit, MI, 48202, USA., Pulido JS; Wills Eye Hospital and Jefferson University Hospital, Philadelphia, PA, 19107, USA., Filler SG; Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, 1124 W Carson St, Torrance, CA, 90502, USA.; David Geffen School of Medicine at UCLA, 10833 Le Conte Ave, Los Angeles, CA, 90095, USA., Mitchell AP; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA.; Department of Microbiology, University of Georgia, 210 S Jackson street, Athens, GA, 30602, USA., Hiller NL; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA., Lacy-Hulbert A; Benaroya Research Institute at Virginia Mason, 1201 9th Ave, Seattle, WA, 98101, USA., Zhao Y; Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2023 Oct; Vol. 10 (30), pp. e2302249. Date of Electronic Publication: 2023 Sep 01.
DOI: 10.1002/advs.202302249
Abstrakt: Super-resolution optical imaging tools are crucial in microbiology to understand the complex structures and behavior of microorganisms such as bacteria, fungi, and viruses. However, the capabilities of these tools, particularly when it comes to imaging pathogens and infected tissues, remain limited. MicroMagnify (µMagnify) is developed, a nanoscale multiplexed imaging method for pathogens and infected tissues that are derived from an expansion microscopy technique with a universal biomolecular anchor. The combination of heat denaturation and enzyme cocktails essential is found for robust cell wall digestion and expansion of microbial cells and infected tissues without distortion. µMagnify efficiently retains biomolecules suitable for high-plex fluorescence imaging with nanoscale precision. It demonstrates up to eightfold expansion with µMagnify on a broad range of pathogen-containing specimens, including bacterial and fungal biofilms, infected culture cells, fungus-infected mouse tone, and formalin-fixed paraffin-embedded human cornea infected by various pathogens. Additionally, an associated virtual reality tool is developed to facilitate the visualization and navigation of complex 3D images generated by this method in an immersive environment allowing collaborative exploration among researchers worldwide. µMagnify is a valuable imaging platform for studying how microbes interact with their host systems and enables the development of new diagnosis strategies against infectious diseases.
(© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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