Towards new fluorometric methodologies based on the in-situ generation of gold nanoclusters.

Autor: Navarro J; Analytical Biosensors Group (GBA), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA), University of Zaragoza-CSIC, C/Pedro Cerbuna 12, 50009, Zaragoza, Spain., Cepriá G; Group of Analytical Spectroscopy and Sensors (GEAS), Instituto de Ciencias Ambientales (IUCA), Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, C/Pedro Cerbuna 12, 50009, Zaragoza, Spain., Camacho-Aguayo J; Analytical Biosensors Group (GBA), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA), University of Zaragoza-CSIC, C/Pedro Cerbuna 12, 50009, Zaragoza, Spain., Martín S; Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, Departamento de Química Física, Universidad de Zaragoza, 50009, Zaragoza, Spain., González Orive A; Department of Chemistry, Materials and Nanotechnology Institute, University of La Laguna, Avda. Astrofísico Francisco Sánchez s/n, 38206, San Cristóbal de La Laguna, Spain., de Marcos S; Analytical Biosensors Group (GBA), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA), University of Zaragoza-CSIC, C/Pedro Cerbuna 12, 50009, Zaragoza, Spain., Galbán J; Analytical Biosensors Group (GBA), Analytical Chemistry Department, Faculty of Sciences, Instituto de Nanociencia y Materiales de Aragón (INMA), University of Zaragoza-CSIC, C/Pedro Cerbuna 12, 50009, Zaragoza, Spain. Electronic address: jgalban@unizar.es.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Jan 01; Vol. 266 (Pt 2), pp. 125119. Date of Electronic Publication: 2023 Aug 24.
DOI: 10.1016/j.talanta.2023.125119
Abstrakt: In this manuscript a method for the fluorometric determination of tyramine is described. It is based on the direct reaction between Au(III) and tyramine in a phosphate buffer which produces fluorescent gold nanoclusters (AuNC) (λ exc  = 320 nm, λ em  = 410 nm) with a diameter of 1.50 ± 0.06 nm. The Au(III) and buffer solutions are mixed and after 140 s, tyramine solution is added; which produces a fast and stable fluorescence signal. The formation of AuNC is demonstrated by STEM and, more importantly, this reaction could be followed by Atomic Fluorescence Microscopy (AFM). The method allows the determination of tyramine in the range from 6.0x10 -7  M (limit of quantification) up to 1.2x10 -4  M; with a relative standard deviation (RSD) ranges from 1.8% to 4.4% depending on the tyramine concentration. The mechanism of AuNC formation involves the Au(III) reduction via the phenol group and the complexation with the amine group. Putrescine and cadaverine do not produce interference, meanwhile histamine causes a proportional decrease in the signal which can be overcome by the standard addition method. The method was applied to the determination of tyramine in a tuna and cheese samples and the results obtained are in statistical agreement with these obtained using a validated or standard method.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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