Evidence of Mycobacterium avium subsp. paratuberculosis binding to albumin: technical and biological implications.

Autor: Jolly A; Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Inmunología, (0054) 11-5287-2155, Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires C1427CWO, Buenos Aires, Argentina. ajolly@fvet.uba.ar., Barnech ML; Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Inmunología, (0054) 11-5287-2155, Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires C1427CWO, Buenos Aires, Argentina.; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina., Duarte JJ; Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Inmunología, (0054) 11-5287-2155, Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires C1427CWO, Buenos Aires, Argentina., Suhevic J; Escuela de Educación Técnico Profesional de nivel medio en Producción Agropecuaria y Agroalimentaria, Universidad de Buenos Aires, Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires C1427CWO, Buenos Aires, Argentina., Jar AM; Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Inmunología, (0054) 11-5287-2155, Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires C1427CWO, Buenos Aires, Argentina., Mundo SL; Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Cátedra de Inmunología, (0054) 11-5287-2155, Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires C1427CWO, Buenos Aires, Argentina.; Universidad de Buenos Aires - CONICET, Instituto de Investigaciones en Producción Animal (INPA), Av. Chorroarín 280, Ciudad Autónoma de Buenos Aires C1427CWO, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2024 Feb; Vol. 48 (1), pp. 271-278. Date of Electronic Publication: 2023 Sep 01.
DOI: 10.1007/s11259-023-10192-0
Abstrakt: Albumin binding ability is a well-characterized feature of many bacteria. To the best of our knowledge, there are no previous reports about this ability among mycobacteria, even when bovine serum albumin (BSA) is a common component of supplements used for the enrichment of synthetic media for mycobacterial growth in vitro and also of buffers used in laboratory techniques. In this work we explored the albumin binding ability of Mycobacterium avium subsp. paratuberculosis (MAP), a pathogenic bacterium causing a known and relevant ruminant disease worldwide, by immunizing rabbits with MAP (grown in media containing or not BSA) or BSA and conducting ELISA and immunoblot experiments with the obtained sera. As a result, we found that MAP can bind BSA when cultured in a conventional BSA-containing medium and when incubated for a short time in the presence of the protein. We also evaluated the host specificity of MAP interaction with albumin and found a preference for the protein of bovine origin when compared with its horse and rabbit homologs. Considerations about its technical and biological implications are discussed.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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