Mouse sperm energy restriction and recovery (SER) revealed novel metabolic pathways.

Autor:	Romarowski A; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, United States.; Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, Argentina., Fejzo J; Institute for Applied Life Sciences, University of Massachusetts, Amherst, MA, United States., Nayyab S; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, United States., Martin-Hidalgo D; Hospital San Pedro de Alcántara, Cáceres, Spain., Gervasi MG; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, United States., Balbach M; Department of Pharmacology, Weill Cornell Medicine, New York, NY, United States., Violante S; Memorial Sloan Kettering Cancer Center, New York, NY, United States., Salicioni AM; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, United States., Cross J; Memorial Sloan Kettering Cancer Center, New York, NY, United States., Levin LR; Department of Pharmacology, Weill Cornell Medicine, New York, NY, United States., Buck J; Department of Pharmacology, Weill Cornell Medicine, New York, NY, United States., Visconti PE; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in cell and developmental biology [Front Cell Dev Biol] 2023 Aug 15; Vol. 11, pp. 1234221. Date of Electronic Publication: 2023 Aug 15 (Print Publication: 2023).
DOI:	10.3389/fcell.2023.1234221
Abstrakt:	Mammalian sperm must undergo capacitation to become fertilization-competent. While working on mice, we recently developed a new methodology for treating sperm in vitro, which results in higher rates of fertilization and embryo development after in vitro fertilization. Sperm incubated in media devoid of nutrients lose motility, although they remain viable. Upon re-adding energy substrates, sperm resume motility and become capacitated with improved functionality. Here, we explore how sperm energy restriction and recovery (SER) treatment affects sperm metabolism and capacitation-associated signaling. Using extracellular flux analysis and metabolite profiling and tracing via nuclear magnetic resonance (NMR) and mass spectrometry (MS), we found that the levels of many metabolites were altered during the starvation phase of SER. Of particular interest, two metabolites, AMP and L-carnitine, were significantly increased in energy-restricted sperm. Upon re-addition of glucose and initiation of capacitation, most metabolite levels recovered and closely mimic the levels observed in capacitating sperm that have not undergone starvation. In both control and SER-treated sperm, incubation under capacitating conditions upregulated glycolysis and oxidative phosphorylation. However, ATP levels were diminished, presumably reflecting the increased energy consumption during capacitation. Flux data following the fate of 13 C glucose indicate that, similar to other cells with high glucose consumption rates, pyruvate is converted into 13 C-lactate and, with lower efficiency, into 13 C-acetate, which are then released into the incubation media. Furthermore, our metabolic flux data show that exogenously supplied glucose is converted into citrate, providing evidence that in sperm cells, as in somatic cells, glycolytic products can be converted into Krebs cycle metabolites. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Romarowski, Fejzo, Nayyab, Martin-Hidalgo, Gervasi, Balbach, Violante, Salicioni, Cross, Levin, Buck and Visconti.)
Databáze:	MEDLINE
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