Nano eggshell-titanium-dioxide biocomposite in occluding opened dentine tubules.

Autor:	Mann NS; Department of Conservative Dentistry and Endodontics, National Dental College, Derabassi, Mohali, Punjab, India., Jhamb A; Department of Conservative Dentistry and Endodontics, National Dental College, Derabassi, Mohali, Punjab, India., Kaur N; Department of Conservative Dentistry and Endodontics, National Dental College, Derabassi, Mohali, Punjab, India., Mann N; Department of Conservative Dentistry and Endodontics, National Dental College, Derabassi, Mohali, Punjab, India.
Jazyk:	angličtina
Zdroj:	Bioinformation [Bioinformation] 2022 Oct 31; Vol. 18 (10), pp. 853-857. Date of Electronic Publication: 2022 Oct 31 (Print Publication: 2022).
DOI:	10.6026/97320630018853
Abstrakt:	It is of interest to synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; and Group 4: GIC mousse. These were then, agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine pre-treatment and post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100µg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability. (© 2022 Biomedical Informatics.)
Databáze:	MEDLINE
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