Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses.

Autor: Vidal Moreno de Vega C; Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Lemmens D; Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., de Meeûs d'Argenteuil C; Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Boshuizen B; Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.; Wolvega Equine Hospital, Oldeholtpade, Netherlands., de Maré L; Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium., Leybaert L; Department of Basic and Applied Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium., Goethals K; Biometrics Research Center, Ghent University, Ghent, Belgium., de Oliveira JE; Cargill R&D Centre Europe, Vilvoorde, Belgium., Hosotani G; Cargill R&D Centre Europe, Vilvoorde, Belgium., Deforce D; Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium., Van Nieuwerburgh F; Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium., Devisscher L; Gut-Liver Immunopharmacology Unit, Department of Basic and Applied Medical Sciences, Liver Research Center Ghent, Ghent University, Ghent, Belgium., Delesalle C; Department of Translational Physiology, Infectiology and Public Health, Research Group of Comparative Physiology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Jazyk: angličtina
Zdroj: Frontiers in physiology [Front Physiol] 2023 Aug 16; Vol. 14, pp. 1256217. Date of Electronic Publication: 2023 Aug 16 (Print Publication: 2023).
DOI: 10.3389/fphys.2023.1256217
Abstrakt: Important changes in glucose transporter (GLUT) expression should be expected if the glucose influx plays a pivotal role in fuelling or connecting metabolic pathways that are upregulated in response to exercise. The aim was to assess GLUT4, 8, and 12 dynamics in response to training and acute exercise. Methods: Sixteen untrained Standardbred mares (3-4 year) performed an incremental SET at the start and end of 8 weeks harness training. M. pectoralis (PM) and M. vastus lateralis (VL) muscle biopsies were taken before and after each SET, allowing for comparing rest and acute samples in untrained (UT) and trained (T) condition using Western Blot for GLUT quantification and Image Pro v.10 for Blot analysis. Data were normalized against GAPDH. Basal GLUT-levels of PM versus VL were analysed with the Wilcoxon matched-pairs signed rank test. The effect of acute exercise or training was assessed using the Friedman test with a post hoc Dunn's. Results: Basal GLUT4 and GLUT12 protein expression were significantly higher in the VL compared to the PM (P GLUT4 = 0.031 and P GLUT12 = 0.002). Training had no effect on basal GLUT4 expression, neither in the VL ( p > 0.9999), nor the PM ( p > 0.9999). However, acute exercise in trained condition significantly decreased GLUT4 expression in the VL ( p = 0.0148). Neither training nor acute exercise significantly changed total GLUT8 protein expression. Training significantly decreased total GLUT12 protein expression in rest biopsies, only visible in the VL ( p = 0.0359). This decrease was even more prominent in the VL after acute exercise in trained condition (P VL = 0.0025). Conclusion: The important changes seen in GLUT12 expression downregulation, both in response to training and acute exercise in the horse, the downregulation of GLUT4 expression after acute exercise in trained condition and the lack of differential shifts in GLUT8 expression in any of the studied conditions, questions the importance of glucose as substrate to fuel training and exercise in healthy horses. These findings encourage to further explore alternative fuels for their involvement in equine muscular energetics.
Competing Interests: JO and GH were employed by the Cargill R&D Centre Europe. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Vidal Moreno de Vega, Lemmens, de Meeûs d’Argenteuil, Boshuizen, de Maré, Leybaert, Goethals, de Oliveira, Hosotani, Deforce, Van Nieuwerburgh, Devisscher and Delesalle.)
Databáze: MEDLINE
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