In vitro Cytotoxicity Evaluation of Flowable Hyaluronic Acid-Acellular Stromal Vascular Fraction (HA-aSVF) Mixture for Tissue Engineering Applications.

Autor:	Mohan SP; Department of Oral and Maxillofacial Pathology, Sree Anjaneya Institute of Dental Sciences, Kozhikode, Kerala, India.; Centre of Stem Cells and Regenerative Medicine, Malabar Medical College Hospital and Research Centre, Kozhikode, Kerala, India., Palaniappan A; Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Vellore, Tamil Nadu, India., Nawaz MKK; Centre of Stem Cells and Regenerative Medicine, Malabar Medical College Hospital and Research Centre, Kozhikode, Kerala, India., Kripamol R; Division of Tissue Culture, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India., Seenuvasan R; Division of Tissue Culture, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India., Kumar PRA; Division of Tissue Culture, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.
Jazyk:	angličtina
Zdroj:	Journal of pharmacy & bioallied sciences [J Pharm Bioallied Sci] 2023 Jul; Vol. 15 (Suppl 1), pp. S677-S682. Date of Electronic Publication: 2023 Jul 05.
DOI:	10.4103/jpbs.jpbs_13_23
Abstrakt:	Background: The stromal vascular fraction (SVF) is an aqueous fraction isolated from the adipose tissue that constitutes different kinds of cells and extracellular matrix components. Hyaluronic acid (HA) is a linear polysaccharide in vertebrate tissues and is considered a potential tissue engineering scaffold due to its biocompatible nature. In this study, we have evaluated the cytotoxicity of xenofree HA in combination with an acellular component of adipose SVF (HA-aSVF) to propose it as a candidate biomaterial for future applications. Materials and Methods: 3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide assay of L-929 cells treated with HA-aSVF was used in our study. Data were normalized to cell control (untreated) and extracts of copper and ultra-high molecular weight polyethylene were used as positive (PC) and negative controls (NC). Results: Fibroblast cells retained the morphology after 24 h of treatment with HA-aSVF mixture and exhibited a similar percentage of cell activity compared to NC. PC showed a positive cytotoxic response as expected. The cells incubated with HA-aSVF showed a linear increase in cell activity indicating proliferation. Conclusion: The mixture of HA and acellular SVF in its flowable form is non-cytotoxic and showed improved cell proliferation. Hence the mixture can be proposed as a biomaterial and can be further explored for specific tissue engineering applications. Competing Interests: There are no conflicts of interest. (Copyright: © 2023 Journal of Pharmacy and Bioallied Sciences.)
Databáze:	MEDLINE
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