Whey and Buttermilk-Based Formulas Modulate Gut Microbiota in Mice with Antibiotic-Induced Dysbiosis.

Autor: Bellés A; Departamento de Farmacología, Fisiología y Medicina Legal y Forense, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, 50013, Spain.; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, 50013, Spain., Abad I; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, 50013, Spain.; Departamento de Producción Animal y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, 50013, Spain., Sánchez L; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, 50013, Spain.; Departamento de Producción Animal y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, 50013, Spain., Grasa L; Departamento de Farmacología, Fisiología y Medicina Legal y Forense, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, 50013, Spain.; Instituto Agroalimentario de Aragón IA2 (UNIZAR-CITA), Zaragoza, 50013, Spain.; Instituto de Investigación Sanitaria de Aragón (IIS Aragón), Zaragoza, 50009, Spain.
Jazyk: angličtina
Zdroj: Molecular nutrition & food research [Mol Nutr Food Res] 2023 Oct; Vol. 67 (20), pp. e2300248. Date of Electronic Publication: 2023 Aug 31.
DOI: 10.1002/mnfr.202300248
Abstrakt: Scope: Diet is one of the main factors that modifies intestinal microbiota composition. The search for foods that can reverse situations of intestinal dysbiosis such as that induced by antibiotics is of great interest. Buttermilk and whey are the main by-products produced by the dairy industry containing bioactive compounds. The aim of this study is to investigate the ability of whey and buttermilk-based formulas supplemented with lactoferrin and milk fat globule membrane (MFGM) to modulate the effects of clindamycin on mouse intestinal microbiota.
Methods and Results: Male C57BL/6 mice are treated with saline (control), clindamycin (Clin), a formula containing whey (F1) or buttermilk (F2), Clin+F1 or Clin+F2, and their fecal microbiota profiles are analyzed by sequencing of 16S rRNA gene using the MinION device. Clin induces alterations in both the composition and metabolic functions of the mice intestinal microbiota. The treatment with F1 or F2 reverses the effects of clindamycin, restoring the levels of Rikenellaceae and Lactobacillaceae families and certain pathways related to short-chain fatty acids production and tetrahydrofolate biosynthesis.
Conclusion: Whey and buttermilk supplemented with lactoferrin and MFGM may be a bioactive formula for functional foods to prevent or restore microbiota alterations induced by antibiotic administration.
(© 2023 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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