Comparison of Phytochemical Composition and Untargeted Metabolomic Analysis of an Extract from Cnidoscolus aconitifolius (Mill.) I. I. Johnst and Porophyllum ruderale (Jacq.) Cass. and Biological Cytotoxic and Antiproliferative Activity In Vitro.

Autor:	Vargas-Madriz ÁF; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico., Luzardo-Ocampo I; Tecnologico de Monterrey, The Institute for Obesity Research, Ave. Eugenio Garza Sada 2501 Sur, Monterrey 64849, Mexico.; Tecnologico de Monterrey, School of Engineering and Science, Campus Guadalajara, Av. General Ramon Corona 2514, Zapopan 45201, Mexico., Moreno-Celis U; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico., Roldán-Padrón O; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico., Chávez-Servín JL; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico., Vergara-Castañeda HA; Advanced Biomedical Research Center, School of Medicine, Universidad Autonoma de Queretaro, Querétaro 76010, Mexico., Martínez-Pacheco M; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico.; Laboratorio de Biomedicina Interdisciplinaria, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico., Mejía C; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico., García-Gasca T; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico., Kuri-García A; Department of Cell and Molecular Biology, School of Natural Sciences, Universidad Autonoma de Queretaro, Querétaro 76230, Mexico.
Jazyk:	angličtina
Zdroj:	Plants (Basel, Switzerland) [Plants (Basel)] 2023 May 15; Vol. 12 (10). Date of Electronic Publication: 2023 May 15.
DOI:	10.3390/plants12101987
Abstrakt:	Cnidoscolus aconitifolius (CA) and Porophyllum ruderale (PR) are representative edible plants that are a traditional food source in Mexico. This research aimed to analyze the phytochemical composition and untargeted metabolomics analysis of CA and PR and evaluate their antiproliferative effect in vitro. The phytochemical composition (UPLC-DAD-QToF/MS-ESI) identified up to 38 polyphenols and selected organic acids that were clustered by the untargeted metabolomics in functional activities linked to indolizidines, pyridines, and organic acids. Compared with PR, CA displayed a higher reduction in the metabolic activity of human SW480 colon adenocarcinoma cells (LC 50 : 10.65 mg/mL), and both extracts increased the total apoptotic cells and arrested cell cycle at G0/G1 phase. PR increased mRNA Apc gene expression, whereas both extracts reduced mRNA Kras expression. Rutin/epigallocatechin gallate displayed the highest affinity to APC and K-RAS proteins in silico. Further research is needed to experiment on other cell lines. Results suggested that CA and PR are polyphenol-rich plant sources exhibiting antiproliferative effects in vitro.
Databáze:	MEDLINE
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