Potential protein hydrolysates from the white and purple flower varieties of Orthosiphon aristatus leaves.

Autor: Chua LS; Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, Malaysia; Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, Malaysia. Electronic address: chualeesuan@utm.my., Abdullah FI; International Institute of Aquaculture and Aquatic Sciences, 71050 Sri Rusa, Port Dickson, Negeri Sembilan, Malaysia., Lim TK; Protein and Proteomics Centre, Department of Biological Sciences, National University of Singapore, 117543, Singapore., Lin Q; Protein and Proteomics Centre, Department of Biological Sciences, National University of Singapore, 117543, Singapore.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Jan 30; Vol. 432, pp. 137261. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1016/j.foodchem.2023.137261
Abstrakt: This study was aimed to extract bioactive peptides from the white and purple flower varieties of Orthosiphon aristatus leaves. The herb is well known for its pharmacological importance, possibly attributed to its plant proteins. Phenol based extraction was used to extract plant proteins, and then hydrolysed by proteolytic enzymes such as trypsin (serine protease) and pepsin (aspartic protease). MS/MS analysis revealed that 145 and 125 proteins were detected from the white and purple flower varieties, respectively. Trypsin hydrolysates were showed to have a higher degree of hydrolysis (24-33%), resulting in higher antioxidant and antibacterial activities. The white flower of trypsin hydrolysates showed a higher radical scavenging activity which could be attributed to its higher content of stress proteins (19%). However, trypsin hydrolysates from the purple flower showed higher ferric reducing power and bacterial growth inhibition. The performance of hydrolysates was better than ampicillin in inhibiting Acinetobacter baumanni and Staphylococcus aureus.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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