Survival of beneficial microbes in liquid bioformulation and optimization of different carrier materials using RSM technique.

Autor: Chompa SS; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Zuan ATK; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. tkz@upm.edu.my., Amin AM; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Hun TG; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Ghazali AHA; School of Biological Sciences, Universiti Sains Malaysia, Gelugor, 11800, Penang, Malaysia., Sadeq BM; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Akter A; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia., Rahman ME; Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.; Soil Resource Development Institute, Divisional Laboratory, Krishi Khamar Sarak, Farmgate, 1215, Dhaka, Bangladesh., Rashid HO; Department of Modern Language & Communications, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Jazyk: angličtina
Zdroj: International microbiology : the official journal of the Spanish Society for Microbiology [Int Microbiol] 2024 Jun; Vol. 27 (3), pp. 697-706. Date of Electronic Publication: 2023 Aug 31.
DOI: 10.1007/s10123-023-00423-4
Abstrakt: Soil salinity in rice cultivation areas is considered a severely limiting factor that adversely affects the quantity and quality of rice production in wetlands. Recently, the alternative use of salt-tolerant plant growth-promoting rhizobacteria (PGPR) inhabiting extreme saline conditions has gained remarkable attention and had positive effects on soil and crops. Therefore, a study has been initiated to develop a liquid biofertilizer formulation from locally isolated multi-strain salt-tolerant PGPR strains such as Bacillus tequilensis and Bacillus aryabhattai, using glycerol (5 mM), trehalose (10 mM), and polyvinylpyrrolidone (PVP) at 1% as additives to prolong the shelf-life of the bacteria. After 3 months of incubation, the bacterial population in the trehalose-supplemented mixed strain was highest at 9.73×10 7 CFU/mL, followed by UPMRE6 and UPMRB9 at 9.40×10 7 CFU/mL and 8.50×10 7 CFU/mL respectively. The results showed that the optimal trehalose concentration successfully prolonged the shelf-life of bacteria with minimal cell loss. Validation of quadratic optimization by response surface methodology revealed that the cell density of the mixed strain was 4.278×10 7 log CFU/mL after 24 h. The precision ratio was 99.7% higher than the predicted value in the minimized medium formulation: 0.267 g/mL trehalose, 1% glycerol, at 120 rpm agitation using the data analysis tools of Design Expert software. The population study confirmed the better and longer survival of salt-tolerant PGPR fortified with 10 mM trehalose, which was considered the best liquid biofertilizer formulation. Moreover, the optimized trehalose-glycerol liquid formulation can be used commercially as it is cost-effective.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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