Immulina as an Immunostimulatory Supplement: Formulation and Pharmacological Studies.

Autor:	Li Y; Department of Pharmaceutics and Drug Delivery, University of Mississippi, University, Mississippi, USA., Ji N; Department of Pharmaceutics and Drug Delivery, University of Mississippi, University, Mississippi, USA., Wang M; Department of Pharmaceutics and Drug Delivery, University of Mississippi, University, Mississippi, USA., Pugh ND; National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, Mississippi, USA., Khan IA; National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, University of Mississippi, University, Mississippi, USA.; Department of BioMolecular Sciences, University of Mississippi, University, Mississippi, USA., Tan C; Department of Pharmaceutical Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
Jazyk:	angličtina
Zdroj:	Planta medica [Planta Med] 2023 Dec; Vol. 89 (15), pp. 1483-1492. Date of Electronic Publication: 2023 Aug 30.
DOI:	10.1055/a-2156-4653
Abstrakt:	Immulina is a commercially available extract of Arthrospira platensis enriched with bacterial lipoproteins that acts as a potent Toll-like receptor 2 agonist. However, the immunostimulatory effect of Immulina is not well understood in vivo . Here, to devise an Immulina formulation suitable for in vivo oral gavage dosing, Immulina nanosuspension was prepared and freeze-dried to yield lyophilized nano-Immulina, which had an average particle size of around 300 nm and fully retained the bioactivity as a Toll-like receptor 2 agonist. Compared to the regular Immulina powder, lyophilized nano-Immulina notably accelerated the dissolution in aqueous media. Immulina nanosuspension was found to stimulate the production of proinflammatory cytokines in murine bone marrow-derived dendritic cells and macrophages. The immune response to Immulina was investigated in healthy mice by longitudinally monitoring the phagocytic activity of circulating neutrophils as a surrogate marker. Following daily oral ingestion of Immulina nanosuspension (10 mg/mouse/day), the phagocytic activity of circulating neutrophils was significantly elevated, suggesting an important mechanism for Immulina to enhance innate immunity. Competing Interests: The authors declare that they have no conflict of interest. (Thieme. All rights reserved.)
Databáze:	MEDLINE
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