Senescent CD153+ T Lymphocytes Increase in the Peripheral Blood of Patients with Thromboangiitis Obliterans.

Autor:	Mashhadi N; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Kasraian L; Blood Transfusion Research Center, High Institute for Education and Research, Shiraz, Iran., Ghoddusi Johari H; Department of Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Hosseinzadeh A; Department of Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Kheshtchin N; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Doroudchi M; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.; Immunology Center for Excellence, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Jazyk:	angličtina
Zdroj:	Iranian journal of immunology : IJI [Iran J Immunol] 2023 Sep 01; Vol. 20 (3), pp. 262-275.
DOI:	10.22034/iji.2023.99188.2622
Abstrakt:	Background: Buerger's disease, also known as Thromboangiitis Obliterans (TAO), is a progressive, inflammatory vascular disease with unknown etiology. Objective: To address the degree of T cell immunosenescence in this inflammatory disease, the frequency of senescent T cells expressing CD57 and/or CD153 (CD30L) in patients with TAO. Methods: In this study, nine male cigarette smoker patients with TAO, nine male healthy cigarette smokers, and nine male healthy non-smoker blood donors were enrolled. PBMCs were extracted from the blood of all participants and stored in liquid nitrogen before use. The percentages of senescent T cells were detected by flow cytometry. The results were analyzed using non-parametric statistical tests. Results: The frequencies of senescent CD3+CD4+CD57+CD153+ and CD3+CD4+CD57-CD153+ T cells significantly increased in patients compared with the non-smoker controls (p=0.01 and p=0.04, respectively). The frequency of senescent CD3+CD4-CD57-CD153+ T cells was higher in patients compared with the smoker controls (p=0.02). In patients with TAO, CD57+CD153- cells were more frequent in CD3hiCD4- and CD3hiCD4+ T cells compared with the CD3loCD4- and CD3loCD4+ T cells (p=0.008 and p=0.0002, respectively). Conversely, the frequency of CD57-CD153+ T cells was significantly higher in CD3loCD4- T cells compared with the CD3hiCD4- T cells (p=0.004). The percentage of CD3+CD4+CD57+CD153- T cells correlated negatively with smoking level in smoker controls (p=0.02, Spearman r=-0.80). Conclusion: Elevated frequencies of senescent CD4+CD57+CD153+ and CD4+CD57-CD153+ T cells in patients compared with non-smoker and smoker controls suggest the contribution of immunosenescence in TAO.
Databáze:	MEDLINE
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