A Synopsis of Proteins and Their Purification.
| Autor: | Walls D; School of Biotechnology , Dublin City University, Dublin, Ireland., Cooney G; School of Biotechnology , Dublin City University, Dublin, Ireland., Loughran ST; Department of Life and Health Sciences, School of Health and Science, Dundalk Institute of Technology, Dundalk, Ireland. sinead.loughran@dkit.ie. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2699, pp. 1-14. |
| DOI: | 10.1007/978-1-0716-3362-5_1 |
| Abstrakt: | The goal of protein purification is to separate a specific protein from all other biomolecules. Classical chromatographic procedures have been designed to exploit particular distinguishing features of individual target proteins, such as size, shape, physicochemical properties, and binding affinity. Advances in molecular biology and bioinformatics have positively contributed at every level to the challenge of purifying individual proteins and more recently have led to the development of high-throughput proteomic platforms. In this chapter, a synopsis of advancements in the field of protein chromatography is presented, with reference to the principal tools and resources that are available to assist with protein purification strategies. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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