Filtration-based technologies for isolation, purification and analysis of extracellular vesicles.
| Autor: | Chernyshev VS; National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, 117997, Moscow, Russia. v_chernyshev@oparina4.ru., Yashchenok A; Skoltech Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology Skolkovo Innovation Center, 121205, Moscow, Russia., Ivanov M; National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, 117997, Moscow, Russia. v_chernyshev@oparina4.ru., Silachev DN; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Physical chemistry chemical physics : PCCP [Phys Chem Chem Phys] 2023 Sep 13; Vol. 25 (35), pp. 23344-23357. Date of Electronic Publication: 2023 Sep 13. |
| DOI: | 10.1039/d3cp03129b |
| Abstrakt: | The involvement of extracellular vesicles (EVs) in cellular communication with multifactorial and multifaceted biological activity has generated significant interest, highlighting their potential diagnostic and therapeutic applications. EVs are found in nearly all biological fluids creating a broad spectrum of where potential disease markers can be found for liquid biopsy development and what subtypes can be used for treatment of diseases. Complexity of biological fluids has generated a variety of different approaches for EV isolation and identification that may in one way or another be most optimal for research studies or clinical use. Each approach has its own advantages and disadvantages, significance of which can be evaluated depending on the end goal of the study. One of the methods is based on filtration which has received attention in the past years due its versatility, low cost and other advantages. Introduction of different approaches for EV capture and analysis that are based on filtration gave rise to new subcategories of filtration techniques which are presented in this overview. Miniaturization and combination of filtration-based approaches with microfluidics is also highlighted due its future prospects in healthcare, especially point-of-need technologies. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|