Inflammatory profile of vertically HIV-1 infected adolescents receiving ART in Cameroon: a contribution toward optimal pediatric HIV control strategies.

Autor: Ka'e AC; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy., Nanfack AJ; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Ambada G; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; Faculty of Science, University of Yaounde 1, Yaounde, Cameroon., Santoro MM; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy., Takou D; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Semengue ENJ; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Nka AD; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Bala MLM; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon., Endougou ON; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon., Elong E; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Beloumou G; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Djupsa S; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Gouissi DH; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Fainguem N; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Tchouaket MCT; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; School of Health Sciences, Catholic University of Central Africa, Yaounde, Cameroon., Sosso SM; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Kesseng D; Mother and Child Centre, Chantal BIYA Foundation, Yaounde, Cameroon., Ndongo FA; Mother and Child Centre, Chantal BIYA Foundation, Yaounde, Cameroon.; Division of Operational Health Research, Ministry of Public Health, Yaounde, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Garoua, Garoua, Cameroon., Sonela N; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Kamta ACL; Elisabeth Glaser Pediatric AIDS Foundation (EGPAF), Country-office, Yaoundé, Cameroon.; HIV Management Unit, Mfou District Hospital, Mfou, Cameroon., Tchidjou HK; Amiens University Hospital, Amiens, France., Ndomgue T; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon., Ndiang STM; National Social Welfare Hospital, Yaounde, Cameroon., Nlend AEN; Higher Institute of Medical Technology, Yaounde, Cameroon., Nkenfou CN; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon., Montesano C; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy., Halle-Ekane GE; Faculty of Health Sciences, University of Buea, Buea, Cameroon., Cappelli G; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; National Research Council, Rome, Italy., Tiemessen CT; National Institute for Communicable Diseases and Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa., Colizzi V; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy.; Faculty of Science and Technology, Evangelic University of Cameroon, Bandjoun, Cameroon., Ceccherini-Silberstein F; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy., Perno CF; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; Bambino Gesu Pediactric Hospital, Rome, Italy., Fokam J; Chantal BIYA International Reference Centre for Research on HIV/AIDS Prevention and Management (CIRCB), Yaounde, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.; Faculty of Health Sciences, University of Buea, Buea, Cameroon.
Jazyk: angličtina
Zdroj: Frontiers in immunology [Front Immunol] 2023 Aug 14; Vol. 14, pp. 1239877. Date of Electronic Publication: 2023 Aug 14 (Print Publication: 2023).
DOI: 10.3389/fimmu.2023.1239877
Abstrakt: Antiretroviral therapy (ART) has improved the lifespan of people living with HIV. However, their immune system remains in a state of sustained activation/inflammation, which favors viral replication and depletion of helper T-cells with varying profiles according to ART-response. We herein sought to ascertain the inflammatory profile of adolescents living with perinatal HIV-1 infection (ALPHI) receiving ART in an African context. In this cross-sectional and comparative study among ART-experienced ALPHI in Yaoundé-Cameroon, HIV-1 RNA was measured by Abbott Real-time PCR; CD4 cells were enumerated using flow cytometry; serum cytokines were measured by ELISA; HIV-1 proviral DNA was genotyped by Sanger-sequencing; and archived drug resistance mutations (ADRMs) were interpreted using Stanford HIVdb.v9.0.1. Overall, 73 adolescents were enrolled (60 ALPHI and 13 HIV-1 negative peers) aged 15 (13-18) years; 60.00% were female. ART median duration was 92 (46-123) months; median viral load was 3.99 (3.17-4.66) RNA Log 10 (copies)/mL and median CD4 count was 326 (201-654) cells/mm 3 . As compared to HIV-negative adolescents, TNFα was highly expressed among ALPHI (p<0.01). Following a virological response, inflammatory cytokines (IFNγ and IL-12), anti-inflammatory cytokines (IL-4 and IL-10) and inflammation-related cytokines (IL-6 and IL-1β) were highly expressed with viral suppression (VS) vs. virological failure (VF), while the chemokine CCL3 was highly expressed with VF (p<0.01). Regarding the immune response, the inflammatory cytokine TNFα was highly expressed in those that are immunocompetent (CD4≥500 cell/mm 3 ) vs. immunocompromised (CD4<500 cell/mm 3 ), p ≤ 0.01; while chemokine CCL2 was highly expressed in the immunocompromised (p<0.05). In the presence of ADRMs, IL-4 and CCL3 were highly expressed (p=0.027 and p=0.043 respectively). Among ART-experienced ALPHI in Cameroon, the TNFα cytokine was found to be an inflammatory marker of HIV infection; IFNγ, IL-1β, IL-6, and IL-12 are potential immunological markers of VS and targeting these cytokines in addition to antiretroviral drugs may improve management. Moreover, CCL3 and CCL2 are possible predictors of VF and/or being immunocompromised and could serve as surrogates of poor ART response.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Ka’e, Nanfack, Ambada, Santoro, Takou, Semengue, Nka, Bala, Endougou, Elong, Beloumou, Djupsa, Gouissi, Fainguem, Tchouaket, Sosso, Kesseng, Ndongo, Sonela, Kamta, Tchidjou, Ndomgue, Ndiang, Nlend, Nkenfou, Montesano, Halle-Ekane, Cappelli, Tiemessen, Colizzi, Ceccherini-Silberstein, Perno and Fokam.)
Databáze: MEDLINE
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