Protective and Therapeutic Role of Ginkgo Biloba Extract Through TRPV1 Channels in an in Vitro Alzheimer's Disease Model.
| Autor: | Özşimşek A; Department of Neurology, Medical School of Alanya Alaaddin Keykubat University, Turkey., Övey İS; Department of Physiology, Medical School of Alanya Alaaddin Keykubat University, Turkey., Karaçay E; Department of Neurology, Medical School of Alanya Alaaddin Keykubat University, Turkey., Yuluğ B; Department of Neurology, Medical School of Alanya Alaaddin Keykubat University, Turkey. |
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| Jazyk: | angličtina |
| Zdroj: | Noro psikiyatri arsivi [Noro Psikiyatr Ars] 2023 Aug 05; Vol. 60 (3), pp. 207-213. Date of Electronic Publication: 2023 Aug 05 (Print Publication: 2023). |
| DOI: | 10.29399/npa.28166 |
| Abstrakt: | Introduction: The effect of Ginkgo biloba (GB) on mitochondria-dependent TRPV1 ion channels in neuroblastoma cells was investigated by creating an Alzheimer's disease (AD) model. Methods: Okadaic acid was applied on SH-SY5Y cells to create an AD model. After cellular differentiation, the study was organized with the seven main groups, examining the effect of GB on calcium depended TRPV1 channels in neuroblastoma cells AD, has been established in vitro. Results: The higher Ca 2 + concentration was detected in the GB+AD, AD and AD+GB groups when compared with the control (p<0.001). The Ca 2 + level was lower in GB+AD and AD+GB groups than in the AD group (p<0.001). Also, cytosolic Ca 2 + concentration was lower in the GB+AD than in the AD+GB group (p<0.05), the apoptosis and intracellular reactive oxygen species (ROS) values were higher in the GB+AD, AD and AD+GB groups than in the control (p<0.001). The apoptosis and intracellular ROS values were higher in AD group than in the GB+AD and AD+GB group (p<0.001) and the apoptosis level was higher in AD+GB group than GB+AD group (p<0.001) and the mitochondrial depolarization, caspase 3 and caspase 9 levels were higher in the GB+AD, AD and AD+GB groups when compared to the control group (p<0.001). Also, the values were lower in the GB+AD group, AD group and AD+GB groups when compared with the GB+AD+capsazepine group, AD+capsazepine group and AD+GB+capsazepine respectively (p<0.001). Conclusion: These results show us that GB has a protective effect besides its therapeutic effect in Alzheimer's disease via TRPV1 channel. Competing Interests: Conflicts of Interest: The authors declared that there is no conflict of interest. (Copyright: © 2023 Turkish Neuropsychiatric Society.) |
| Databáze: | MEDLINE |
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