"Slow kill" treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis.

Autor: Rajković M; Department of Parasitology, University of Belgrade - Faculty of Veterinary Medicine, Belgrade 11000, Serbia., Glavinić U; Department of Biology, University of Belgrade - Faculty of Veterinary Medicine, Belgrade 11000, Serbia., Bogunović D; Department of Parasitology, University of Belgrade - Faculty of Veterinary Medicine, Belgrade 11000, Serbia., Vejnović B; Department of Economics and Statistics, University of Belgrade - Faculty of Veterinary Medicine, Belgrade 11000, Serbia. Electronic address: branislavv@vet.bg.ac.rs., Davitkov D; Department of Equine, Small Animal, Poultry and Wild Animal Diseases, University of Belgrade - Faculty of Veterinary Medicine, Belgrade 11000, Serbia., Đelić N; Department of Biology, University of Belgrade - Faculty of Veterinary Medicine, Belgrade 11000, Serbia., Stanimirović Z; Department of Biology, University of Belgrade - Faculty of Veterinary Medicine, Belgrade 11000, Serbia.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2023 Oct; Vol. 322, pp. 110008. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1016/j.vetpar.2023.110008
Abstrakt: Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs' leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of sensitivity to DNA damage in leukocytes treated with H 2 O 2 using the in vivo and ex vivo comet assay. The whole blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote, Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott's test and PCR were used in the aim of detecting D. immitis microfilariae in dogs' blood, and evaluating the number of circulating microfilariae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that leukocytes exhibited decreased sensitivity to H 2 O 2 -induced DNA damage during treatment. The results of the modified Knott's test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from the dogs' blood.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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