Modelling Duchenne muscular dystrophy in vitro with newly generated, blood cell-derived induced pluripotent stem cell line ORIONi003-A.

Autor:	Hajduchova D; Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Suroviakova S; Department of Pediatric Neurology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, and University Hospital Martin, Slovakia., Mersakova S; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Brany D; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Zahumenska R; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Rehak M; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Skovierova H; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Nováková S; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Nosal V; Clinic of Neurology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, and University Hospital Martin, Martin, Slovakia., Marcinek J; Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, and University Hospital Martin, Slovakia., Kalman M; Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, and University Hospital Martin, Slovakia., Jozef Pec M; Department of Internal Medicine I, University Hospital Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Brozmanova M; Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia; Central Animal Facility, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Melegova J; Central Animal Facility, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Juhas S; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i, Liběchov, Czech Republic., Juhasova J; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, v.v.i, Liběchov, Czech Republic., Studenovska H; Department of Biomaterials and Bioanalogous Systems Institute of Macromolecular Chemistry Czech Academy of Sciences, Prague, Czech Republic., Mitruskova B; Department of Clinical Genetics, Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, and University Hospital, Martin, Slovakia., Pokusa M; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Samec M; Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Samos M; Department of Internal Medicine I, University Hospital Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Nicodemou A; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Slovakia., Danisovic L; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Slovakia., Dankova Z; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Kurca E; Clinic of Neurology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, and University Hospital Martin, Martin, Slovakia., Lexova Kolejakova K; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Slovakia., Chandoga J; Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Slovakia., Plank L; Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, and University Hospital Martin, Slovakia., Halasova E; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Pecova R; Department of Pathological Physiology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia., Strnadel J; Biomedical Centre Martin JFM CU, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Slovakia. Electronic address: jan.strnadel@uniba.sk.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2023 Sep; Vol. 71, pp. 103187. Date of Electronic Publication: 2023 Aug 22.
DOI:	10.1016/j.scr.2023.103187
Abstrakt:	Here, we present newly derived in vitro model for modeling Duchenne muscular dystrophy. Our new cell line was derived by reprogramming of peripheral blood mononuclear cells (isolated from blood from pediatric patient) with Sendai virus encoding Yamanaka factors. Derived iPS cells are capable to differentiate in vitro into three germ layers as verified by immunocytochemistry. When differentiated in special medium, our iPSc formed spontaneously beating cardiomyocytes. As cardiomyopathy is the main clinical complication in patients with Duchenne muscular dystrophy, the cell line bearing the dystrophin gene mutation might be of interest to the research community. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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