Quantification of Bioactive Metabolites Derived from Cell-Free Supernatant of Pediococcus acidilactici and Screening their Protective Properties in Frankfurters.

Autor: İncili GK; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Fırat University, Elazıg, Turkey. gkincilli@firat.edu.tr., Akgöl M; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Fırat University, Elazıg, Turkey., Karatepe P; Food Processing Department, Keban Vocational School, Fırat University, Elazıg, Turkey., Üner S; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Siirt University, Siirt, Turkey., Tekin A; Food Processing Department, Keban Vocational School, Fırat University, Elazıg, Turkey., Kanmaz H; Department of Food Engineering, Engineering Faculty, Inonu University, Malatya, Turkey., Kaya B; Department of Food Engineering, Engineering Faculty, Inonu University, Malatya, Turkey., Çalicioğlu M; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Fırat University, Elazıg, Turkey., Hayaloğlu AA; Department of Food Engineering, Engineering Faculty, Inonu University, Malatya, Turkey.
Jazyk: angličtina
Zdroj: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2023 Aug 29. Date of Electronic Publication: 2023 Aug 29.
DOI: 10.1007/s12602-023-10147-6
Abstrakt: The specific aims of the current study were to determine and quantify the bioactive compounds derived from the cell-free supernatant (CFS) of Pediococcus acidilactici and screen their protective effect in frankfurters by applying an edible coating. This was achieved by immersing the peeled frankfurters in the CFS (CFS: 50% and 100%) alone or in combination with chitosan (CH: 0.5% and 1%) solutions for 3 min. Untreated frankfurter samples (control) exceeded the maximum acceptable total viable count limit (7.0 log 10 ) on the 14th day, whereas samples treated with 100% CFS + 1% chitosan reached the limit on day 28 during refrigerated storage (P < 0.05). This treatment provided a 14-day extension to the shelf life of frankfurters without causing any significant changes in color and sensory attributes (P > 0.05). Additionally, this treatment inhibited oxidation in the frankfurters, leading to no significant changes in TBA and TVB-N within this group during storage (P > 0.05). This protective effect was mainly attributed to the wide variety of bioactive compounds identified in the CFS, including a total of 5 organic acids, 20 free amino acids, 11 free fatty acids, 77 volatiles, and 10 polyphenols. Due to these bioactive compounds, CFS exhibited a strong radical scavenging capacity (DPPH: 435.08 TEAC/L, ABTS: 75.01 ± 0.14 mg TEAC/L; FRAP: 1.30 ± 0.03 mM FE/L) and antimicrobial activity against microorganisms primarily responsible for the spoilage of frankfurters. In conclusion, the results indicate that the CFS contains high levels of bioactive metabolites, and an edible chitosan coating impregnated with CFS can be utilized to extend the shelf life of frankfurters through its antimicrobial effects and oxidation stabilization.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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