Antitumor and antibacterial activity of metabolites of endophytic Colletotrichum siamense isolated from coffee (Coffea arabica L. cv IAPAR-59).

Autor: do Espírito Santo BC; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil., Oliveira JADS; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil., Ribeiro MADS; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil., Schoffen RP; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil., Polli AD; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil., Polonio JC; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil. jcpolonio2@uem.br., da Silva AA; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil., de Abreu Filho BA; Center for Health Sciences, Department of Basic Health Sciences, Universidade Estadual de Maringá, Maringá, PR, Brazil., Heck MC; Department of Biotechnology, Genetics and Cell Biology, Universidade Estadual de Maringá, Maringá, PR, Brazil., Meurer EC; LabFenn, Universidade Federal Do Paraná, Jandaia Do Sul, Brazil., Constantin PP; Department of Structural, Molecular and Genetic Biology, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil., Pileggi M; Department of Structural, Molecular and Genetic Biology, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil., Vicentini VEP; Department of Biotechnology, Genetics and Cell Biology, Universidade Estadual de Maringá, Maringá, PR, Brazil., Golias HC; Department of Biotechnology, Genetics and Cell Biology, LBioMic, Universidade Estadual de Maringá, Maringá, PR, Brazil., Pamphile JA
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Dec; Vol. 54 (4), pp. 2651-2661. Date of Electronic Publication: 2023 Aug 29.
DOI: 10.1007/s42770-023-01104-0
Abstrakt: Endophytic fungi produce a range of known metabolites and several others, not yet explored, which present important biological activities from the pharmaceutical and industrial perspective. Several studies have reported the diversity of endophytes in Coffea arabica plants, although few have been described in organic cultures. In the current paper, we describe the chemical profile of specialized metabolites in the ethyl acetate phase in a strain of the endophytic fungus Colletotrichum siamense associated with coffee (Coffea arabica L.) (Rubiaceae) and its potential against tumor cells and bacteria of medical and food importance. Cytotoxicity assays in tumor cells MCF-7 and HepG2/C3A were performed by MTT and microdilution in broth to evaluate the antibacterial action of metabolic extract. The antiproliferative assay showed promising results after 24 h of treatment, with 50% injunction concentrations for the two cell types. UHPLC-MS/MS analyses with an electrospray ionization source were used to analyze the extracts and identify compounds of species Colletotrichum siamense, which is still little explored as a source of active metabolites. Many of these compounds observed in the endophytic need to be chemically synthesized in industry, at high costs, while production by the fungus becomes a chemically and economically more viable alternative. Pyrocatechol, gentisyl alcohol, and alpha-linolenic acid, associated with different mechanisms of action against tumor cells, were detected among the main compounds. The extract of the endophytic fungus Colletotrichum siamense presented several compounds with pharmacological potential and antibacterial activity, corroborating its potential in biotechnological applications.
(© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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