Musa acuminata as electroporation model.

Autor: Andrade DLLS; Institute of Biomedical Engineering, Department of Electrical and Electronics Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil., Pintarelli GB; Department of Control and Automation Engineering, Federal University of Santa Catarina, Blumenau, SC, Brazil., Rosa JV; Institute of Biomedical Engineering, Department of Electrical and Electronics Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil., Paro IB; Institute of Biomedical Engineering, Department of Electrical and Electronics Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil., Pagano PJT; Institute of Biomedical Engineering, Department of Electrical and Electronics Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil., Silva JCN; Institute of Biomedical Engineering, Department of Electrical and Electronics Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil., Suzuki DOH; Institute of Biomedical Engineering, Department of Electrical and Electronics Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2023 Dec; Vol. 154, pp. 108549. Date of Electronic Publication: 2023 Aug 22.
DOI: 10.1016/j.bioelechem.2023.108549
Abstrakt: Electrochemotherapy (ECT) and Irreversible electroporation (IRE) are cancer treatments based on electric field distribution in tissues. Solanum tuberosum (potato tissue) phantom is known to mimic changes in the electrical conductivity that occur in animal tissues during electroporation (EP). Electric field distribution is assessed through enzymatic staining. However, the 24-h wait for this assessment could slow agile response scenarios. We developed and validated the Musa acuminata (cavendish banana) conductivity model, which quickly evaluates EP by tissue staining. We investigated the frequency response of the tissue using impedance spectroscopy analysis, conductivity changes, and enzymatic staining. We optimized three usual EP models: adapted Gompertz, smoothed Heaviside, and the sigmoid or logistic function. We found dielectric parameters in banana tissue similar to those in potato (electrical conductivity of 0.035 S/m and relative permittivity of 4.1×10 4 ). The coefficients of determination R 2 were 99.94% (Gompertz), 99.85% (Heaviside), and 99.58% (sigmoid). The sigmoid and Heaviside functions described the calibration and validation electric currents with 95% confidence. We observed the electroporated areas in bananas 3h30m after EP. Staining was significant after 450 V/cm. The conductivity model of Musa acuminata suits treatment planning, hardware development, and training scenarios. Banana phantom supports the 3Rs practice and is a reliable alternative for potato in EP studies.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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