Comparison of individual and pooled sampling methods for estimation of Vairimorpha ( Nosema ) spp. levels in experimentally infected honey bee colonies.

Autor:	Biganski S; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Lester T; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Obshta O; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Jose MS; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Thebeau JM; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Masood F; Veterinary Microbiology, University of Saskatchewan, Saskatoon, SK, Canada., Silva MCB; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Camilli MP; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Raza MF; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Zabrodski MW; Western College of Veterinary Medicine, and Prairie Diagnostic Services, University of Saskatchewan, Saskatoon, SK, Canada., Kozii I; Western College of Veterinary Medicine, and Prairie Diagnostic Services, University of Saskatchewan, Saskatoon, SK, Canada., Koziy R; Western College of Veterinary Medicine, and Prairie Diagnostic Services, University of Saskatchewan, Saskatoon, SK, Canada., Moshynskyy I; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Simko E; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada., Wood SC; Departments of Veterinary Pathology, University of Saskatchewan, Saskatoon, SK, Canada.
Jazyk:	angličtina
Zdroj:	Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2023 Nov; Vol. 35 (6), pp. 639-644. Date of Electronic Publication: 2023 Aug 28.
DOI:	10.1177/10406387231194620
Abstrakt:	The microsporidian pathogens Vairimorpha apis and V. ceranae are known to cause intestinal infection in honey bees and are associated with decreased colony productivity and colony loss. The widely accepted method for determining Vairimorpha colony infection level for risk assessment and antibiotic treatment is based on spore counts of 60 pooled worker bees using light microscopy. Given that honey bee colonies consist of as many as 1,000 times more individuals, the number of bees collected for Vairimorpha detection may significantly impact the estimated colony infection level, especially in the case of uneven distribution of high- and low-infected individuals within a hive. Hence, we compared the frequency and severity of Vairimorpha infection in individual bees to pooled samples of 60, 120, and 180 bees, as well as compared the Vairimorpha spp. prevalence in pooled samples of 60 and 180 bees. Overall, we did not find significant differences in spore counts in pooled samples containing incremental numbers of bees, although we observed that, in less-infected colonies, a low frequency of highly infected individuals influenced the estimated colony infection level. Moreover, Vairimorpha spp. prevalence did not differ significantly among the pooled bee samples tested. Increasing the number of pooled bees from the recommended 60 bees to 180 bees did not yield a more accurate representation of colony infection level for highly infected colonies, but the clinical importance of a low frequency of highly infected individuals in less-infected colonies needs to be addressed in future studies. Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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