LITE-1 mediates behavioral responses to X-rays in Caenorhabditis elegans .

Autor:	Cannon KE; Department of Vision Sciences, School of Optometry, University of Alabama at Birmingham, Birmingham, AL, United States.; Department of Neurobiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States.; Department of Radiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States., Ranasinghe M; Department of Chemistry, Clemson University, Clemson, SC, United States., Millhouse PW; Department of Chemistry, Clemson University, Clemson, SC, United States., Roychowdhury A; Department of Radiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States., Dobrunz LE; Department of Neurobiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States., Foulger SH; Department of Materials Science and Engineering, College of Engineering, Computing and Applied Sciences, Clemson University, Clemson, SC, United States., Gauntt DM; Department of Radiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States., Anker JN; Department of Chemistry, Clemson University, Clemson, SC, United States., Bolding M; Department of Radiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in neuroscience [Front Neurosci] 2023 Aug 10; Vol. 17, pp. 1210138. Date of Electronic Publication: 2023 Aug 10 (Print Publication: 2023).
DOI:	10.3389/fnins.2023.1210138
Abstrakt:	Rapid sensory detection of X-ray stimulation has been documented across a wide variety of species, but few studies have explored the underlying molecular mechanisms. Here we report the discovery of an acute behavioral avoidance response in wild type Caenorhabditis elegans to X-ray stimulation. The endogenous C. elegans UV-photoreceptor protein LITE-1 was found to mediate the locomotory avoidance response. Transgenic expression of LITE-1 in C. elegans muscle cells resulted in paralysis and egg ejection responses to X-ray stimulation, demonstrating that ectopic expression of LITE-1 can confer X-ray sensitivity to otherwise X-ray insensitive cells. This work represents the first demonstration of rapid X-ray based genetically targeted (X-genetic) manipulation of cellular electrical activity in intact behaving animals. Our findings suggest that LITE-1 has strong potential for use in this minimally invasive form of neuromodulation to transduce transcranial X-ray signals for precise manipulation of neural activity in mammals, bypassing the need for invasive surgical implants to deliver stimulation. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Cannon, Ranasinghe, Millhouse, Roychowdhury, Dobrunz, Foulger, Gauntt, Anker and Bolding.)
Databáze:	MEDLINE
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