Molecular and serological prevalence of Coxiella burnetii in bovine dairy herds in southern Chile: A PCR and ELISA-based assessment of bulk tank milk samples.

Autor: Hernández-Agudelo JM; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile., Bartolotti C; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile., Tejeda C; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile., Tomckowiak C; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile., Soto JP; Prolesur, Los Lagos, Chile., Steuer P; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile., Ulloa F; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile., Salgado M; Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile. Electronic address: miguelsalgado@uach.cl.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2023 Nov; Vol. 247, pp. 107008. Date of Electronic Publication: 2023 Aug 26.
DOI: 10.1016/j.actatropica.2023.107008
Abstrakt: Coxiella burnetii (C. burnetii) is a highly resilient zoonotic bacterium responsible for Q fever, a disease which occurs worldwide, with the exception of New Zealand. However, in Chile, the prevalence and impact of C. burnetii in cattle herds remain poorly understood due to limited research. This study aimed to assess the presence of C. burnetii in dairy cattle herds in southern Chile, using two diagnostic methods on bulk tank milk samples. The results of the study revealed a high prevalence of C. burnetii infection in the analyzed herds. Of the 271 milk tank samples tested, 76% (208/271, CI: 71.1-81.5) tested positive using ELISA, while 73% (200/271, CI: 68.0-78.8) tested positive using qPCR. These findings indicate a significant presence of C. burnetii in the cattle herds studied. Despite the high prevalence observed, no new Q fever outbreaks have been reported in the study area. This discrepancy highlights the need for further research to better understand the transmission dynamics, environmental factors, and livestock management practices associated with C. burnetii infection. These studies will contribute to the development of effective prevention and control strategies and promote public health regarding Q fever.
Competing Interests: Declaration of Competing Interest The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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