HLA-G alleles and their impacts on placental HSV-1 infection in women from southern Brazil.

Autor: Tornatore M; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Amaral SC; Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil., Alves BM; Post-Graduate Program in Oncovirology, Instituto Nacional do Câncer (INCA), Rio de Janeiro, RJ, Brazil., de Oliveira GR; Post-Graduate Program in Oncovirology, Instituto Nacional do Câncer (INCA), Rio de Janeiro, RJ, Brazil., Finger-Jardim F; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Avila EC; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Pivato AF; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Lobato RC; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Chies JAB; Laboratory of Immunobiology and Immunogenetics, Post-Graduate Program in Genetics and Molecular Biology (PPGBM), Department of Genetics, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., Ellwanger JH; Laboratory of Immunobiology and Immunogenetics, Post-Graduate Program in Genetics and Molecular Biology (PPGBM), Department of Genetics, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil., Soares EA; Post-Graduate Program in Oncovirology, Instituto Nacional do Câncer (INCA), Rio de Janeiro, RJ, Brazil., Sánchez-Luquez K; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Gonçalves CV; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Martínez AMB; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil., Soares MA; Post-Graduate Program in Oncovirology, Instituto Nacional do Câncer (INCA), Rio de Janeiro, RJ, Brazil; Department of Genetics, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., da Hora VP; Post-Graduate Program in Health Sciences, Faculty of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil. Electronic address: dahoravp@gmail.com.
Jazyk: angličtina
Zdroj: Journal of reproductive immunology [J Reprod Immunol] 2023 Sep; Vol. 159, pp. 104134. Date of Electronic Publication: 2023 Aug 15.
DOI: 10.1016/j.jri.2023.104134
Abstrakt: The Human Leukocyte Antigen G (HLA-G) is an immunoregulatory molecule with a critical role in pregnancy success. HLA-G alleles are associated with differential susceptibility to multiple conditions, including gestational problems, infectious diseases, and viral persistence. Of note, both herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) can impair HLA-G expression, interfering with HLA-G-associated immunoregulation. On the other hand, the impacts of HLA-G alleles on susceptibility to Herpesviridae infection is a neglected issue. Therefore, this study evaluated HLA-G allele frequencies and their associations with placental Herpesviridae infection in women from southern Brazil. Placenta samples were collected soon after delivery, and detection of viral DNA of HSV-1, HSV-2 and human cytomegalovirus (HCMV) was performed by polymerase chain reaction (PCR). A fragment of HLA-G (exons 2-4) was amplified by PCR, sequenced, and analyzed to allele determination. One hundred and seventy women had their alleles determined. Overall, 25 HLA-G alleles were found, distributed into 56 different genotypes. The most frequent alleles were G* 01:01:01 and G* 01:01:02, found in 37.9 % and 16.5 % of samples, respectively. Among the 170 women, 89 (52.4 %) tested positive for Herpesviridae DNA in the placenta, 55 (32.3 %) tested negative, 3 (1.8 %) were negative for HSV-1 and HSV-2 (with absent HCMV data), and 23 (13.5 %) were undetermined. The G* 01:01:01 allele was significantly associated with an increased risk of placental HSV-1 infection (p = 0.0151; OR=1.837; IC=1.108-3.045). This study describes new information concerning placental HLA-G alleles in women from southern Brazil and helps explain how genetic background can modify susceptibility to placental infections.
Competing Interests: Declaration of Competing Interest The authors declare no conflicts of interest concerning this study.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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