Transforming growth factor beta-3 localization in the corneal response to epithelial-stromal injury and effects on corneal fibroblast transition to myofibroblasts.

Autor: Shiju TM; Cole Eye Institute, Cleveland Clinic, Cleveland, OH, United States., Sampaio LP; Cole Eye Institute, Cleveland Clinic, Cleveland, OH, United States; Department of Ophthalmology at University of Sao Paulo, Sao Paulo, Brazil., Martinez VV; Cole Eye Institute, Cleveland Clinic, Cleveland, OH, United States., Hilgert GSL; Cole Eye Institute, Cleveland Clinic, Cleveland, OH, United States., Wilson SE; Cole Eye Institute, Cleveland Clinic, Cleveland, OH, United States. Electronic address: wilsons4@ccf.org.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2023 Oct; Vol. 235, pp. 109631. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1016/j.exer.2023.109631
Abstrakt: The purpose of this study was to evaluate the localization of TGF beta-3 in situ in unwounded rabbit corneas and corneas that had epithelial-stromal injuries produced by photorefractive keratectomy (PRK) in rabbits and to evaluate the in vitro effects of TGF beta-3 compared to TGF beta-1 on alpha-smooth muscle actin (α-SMA) protein expression and myofibroblast development in corneal fibroblasts. Forty-eight New Zealand white rabbits underwent either -3 diopter (D) or -9D PRK and were studied from one to eight weeks (four corneas in each group at each time point) after surgery with immunohistochemistry for TGF beta-3, laminin alpha-5, and alpha-smooth muscle actin (α-SMA). Rabbit corneal fibroblasts were treated with activated TGF beta-1 and/or TGF beta-3 at different concentrations and duration of exposure and studied with immunocytochemistry for myofibroblast development and the expression of α-SMA using Jess automated Western blotting. TGF beta-3 was detected at high levels in the stroma of unwounded corneas and corneas at one to eight weeks after -3D or -9D PRK, as well as in the epithelium and epithelial basement membrane (EBM). No difference was noted between corneas that healed with and without myofibroblast-mediated fibrosis, although TGF beta-3 was commonly associated with myofibroblasts. TGF beta-3 effects on corneal fibroblasts in vitro were similar to TGF beta-1 in stimulating transition to α-SMA-positive myofibroblasts and promoting α-SMA protein expression. The corneal stromal localization pattern of TGF beta-3 protein in unwounded corneas and corneas after epithelial-stromal injury was found to be higher and different from TGF beta-1 and TGF beta-2 reported in previous studies. TGF beta-3 had similar effects to TGF beta-1 in driving myofibroblast development and α-SMA expression in corneal fibroblasts cultured in medium with 1% fetal bovine serum.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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