The Influence of the Omicron Variant on RNA Extraction and RT-qPCR Detection of SARS-CoV-2 in a Laboratory in Brazil.

Autor:	Silva LM; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Riani LR; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Leite JB; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., de Assis Chagas JM; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Fernandes LS; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Fochat RC; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Perches CGP; Hospital Universitário, Universidade Federal de Juiz de Fora, Av. Eugênio do Nascimento, s/n, Juiz de Fora 36038-330, MG, Brazil., Nascimento TC; Faculdade de Enfermagem, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Jaeger LH; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Silvério MS; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Dos Santos Pereira-Júnior O; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil., Pittella F; Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n-Campus Universitário, Juiz de Fora 36036-900, MG, Brazil.
Jazyk:	angličtina
Zdroj:	Viruses [Viruses] 2023 Aug 04; Vol. 15 (8). Date of Electronic Publication: 2023 Aug 04.
DOI:	10.3390/v15081690
Abstrakt:	The emergence of SARS-CoV-2 variants can affect their detection via RT-qPCR. The Omicron variant has a greater affinity for the upper respiratory system and causes clinical conditions similar to bronchitis, as opposed to the pneumonitis-like conditions caused by other SARS-CoV-2 variants. This characteristic increases the viscosity of clinical samples collected for diagnosis. Coinciding with the arrival of the Omicron variant, we observed a failure in control gene expression in our laboratory. In this report, we have optimized a rapid nucleic acid extraction step to restore gene expression and detect the presence of the SARS-CoV-2 virus. We reevaluated 3000 samples, compared variant types occurring in different time periods, and confirmed that the presence of the Omicron variant was responsible for changes observed in the characteristics of these clinical samples. For samples with large amounts of mucus, such as those containing the Omicron variant, a modification to the nucleic acid extraction step was sufficient to restore the quality of RT-qPCR results.
Databáze:	MEDLINE
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