| Autor: |
Ivanova AA; Federal Research Center 'Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences', 142290 Pushchino, Russia., Sazonova OI; Federal Research Center 'Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences', 142290 Pushchino, Russia., Zvonarev AN; Federal Research Center 'Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences', 142290 Pushchino, Russia., Delegan YA; Federal Research Center 'Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences', 142290 Pushchino, Russia.; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Streletskii RA; Laboratory of Ecological Soil Science, Faculty of Soil Science, Lomonosov Moscow State University, 119991 Moscow, Russia., Shishkina LA; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Bogun AG; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Vetrova AA; Federal Research Center 'Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences', 142290 Pushchino, Russia. |
| Abstrakt: |
The complete genome of the naphthalene- and n -alkane-degrading strain Pseudomonas sp. strain OVF7 was collected and analyzed. Clusters of genes encoding enzymes for the degradation of naphthalene and n -alkanes are localized on the chromosome. Based on the Average Nucleotide Identity and digital DNA-DNA Hybridization compared with type strains of the group of fluorescent pseudomonads, the bacterium studied probably belongs to a new species. Using light, fluorescent, and scanning electron microscopy, the ability of the studied bacterium to form biofilms of different architectures when cultured in liquid mineral medium with different carbon sources, including naphthalene and n -dodecane, was demonstrated. When grown on a mixture of naphthalene and n -dodecane, the strain first consumed naphthalene and then n -dodecane. Cultivation of the strain on n -dodecane was characterized by a long adaptation phase, in contrast to cultivation on naphthalene and a mixture of naphthalene and n -dodecane. |
