Autor: |
Paswan M; Department of Chemistry, Sardar Vallabhbhai National Institute of Technology, Surat 395007, India., Adhikary S; Process Design and Engineering Cell, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 346002, India., Salama HH; National Research Centre, Dairy Department, Food Industries and Nutrition Research Institute, 33 El-Buhouth Str. (Former El-Tahrir Str.), Dokki, Giza 12622, Egypt., Rusu AV; CENCIRA Agrofood Research and Innovation Centre, Ion Meșter 6, 400650 Cluj-Napoca, Romania., Zuorro A; Department of Chemical Engineering, Materials and Environment, Sapienza University of Rome, 00184 Rome, Italy., Dholakiya BZ; Department of Chemistry, Sardar Vallabhbhai National Institute of Technology, Surat 395007, India., Trif M; Food Research Department, Centre for Innovative Process Engineering (CENTIV) GmbH, 28857 Syke, Germany., Bhattacharya S; Process Design and Engineering Cell, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar 346002, India.; Academy of Scientific and Innovative Research (AcSIR), Bhavnagar 364002, India. |
Abstrakt: |
Cotton stalk, a waste product in agriculture, serves as a beneficial, low-cost material as a medium for microbial synthesis of lactic acid as desired for polylactic acid synthesis. Cotton stalk was used as a substrate for microbial lactic acid synthesis, and a novel strain of Lactococcus cremoris was reported to synthesize 51.4 g/L lactic acid using cellulose recovered from the cotton stalk. In total, 18 Lactobacillus isolates were isolated from kitchen waste, soil, sugarcane waste, and raw milk samples screened for maximum lactic acid production. It was found that one of the Lactococcus cremoris isolates was found to synthesize maximum lactic acid at a concentration of 51.4 g/L lactic acid in the hydrolysate prepared from cotton stalk. The upstream process parameters included 10% inoculum size, hydrolysate containing reducing sugars 74.23 g/L, temperature 37 °C, agitation 220 rpm, production age 24 h. Only the racemic (50:50) mixture of D-LA and L-LA (i.e., D/L-LA) is produced during the chemical synthesis of lactic acid, which is undesirable for the food, beverage, pharmaceutical, and biomedical industries because only the L-form is digestible and is not suitable for biopolymer, i.e., PLA-based industry where high optically purified lactic acid is required. Furthermore, polylactic acid was synthesized through direct polycondensation methods using various catalysts such as chitosan, YSZ, and Sb 2 O 3 . PLA is biocompatible and biodegradable in nature (its blends and biocomposites), supporting a low-carbon and circular bioeconomy. |