| Autor: |
Sigolaeva LV; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, Russia., Shalybkova AA; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, Russia., Sharifullin TZ; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, Russia., Pergushov DV; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Micromachines [Micromachines (Basel)] 2023 Aug 18; Vol. 14 (8). Date of Electronic Publication: 2023 Aug 18. |
| DOI: |
10.3390/mi14081629 |
| Abstrakt: |
A novel approach to surface modification, which consists of the adsorption of microgel-enzyme complexes preformed in solution, is highlighted. Accordingly, the microgel-enzyme complexes were formed due to the electrostatic interaction of the oppositely charged interacting components, that is, a cationic poly( N -isopropylacrylamide)-based microgel and glucose oxidase taken as a model enzyme. The spontaneous adsorption of the prepared microgel-enzyme complexes, examined by means of quartz crystal microbalance with dissipation monitoring and atomic force microscopy, was observed, resulting in the formation of well-adhered microgel-enzyme coatings. Further, the preformed microgel-enzyme complexes were adsorbed onto the modified graphite-based screen-printed electrodes, and their enzymatic responses were determined by means of amperometry, demonstrating a remarkable analytical performance toward the quantification of β-D-glucose in terms of high sensitivity (0.0162 A × M -1 × cm -2 ), a low limit of detection (1 μM), and an expanded linear range (1-2000 μM). The fabricated microgel-enzyme biosensor constructs were found to be very stable against manifold-repeated measurements. Finally, the pH- or salt-induced release of glucose oxidase from the adsorbed preformed microgel-enzyme complexes was demonstrated. The findings obtained for the microgel-enzyme coatings prepared via adsorption of the preformed microgel-enzyme complexes were compared to those found for the microgel-enzyme coatings fabricated via a previously exploited two-stage sequential adsorption, which includes the adsorption of the microgel first, followed by the electrostatic binding of glucose oxidase by the adsorbed microgel. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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