| Autor: |
Tan JH; Microbial Cell Bioprocessing, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore 138668, Singapore., Chen A; Chemical Biotechnology and Biocatalysis, Institute of Sustainability for Chemicals, Energy and Environment, Agency for Science, Technology and Research (A*STAR), Singapore 138665, Singapore., Bi J; Molecular Engineering Lab, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138673, Singapore.; Department of Food Science and Technology, National University of Singapore, 2 Science Drive 2, Singapore 117542, Singapore., Lim YH; Chemical Biotechnology and Biocatalysis, Institute of Sustainability for Chemicals, Energy and Environment, Agency for Science, Technology and Research (A*STAR), Singapore 138665, Singapore.; Synthetic Biology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore, 10 Medical Drive, Singapore 117597, Singapore., Wong FT; Chemical Biotechnology and Biocatalysis, Institute of Sustainability for Chemicals, Energy and Environment, Agency for Science, Technology and Research (A*STAR), Singapore 138665, Singapore.; Molecular Engineering Lab, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore 138673, Singapore., Ow DS; Microbial Cell Bioprocessing, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore 138668, Singapore. |
| Abstrakt: |
The rare sugar D -allulose is a potential replacement for sucrose with a wide range of health benefits. Conventional production involves the employment of the Izumoring strategy, which utilises D -allulose 3-epimerase (DAEase) or D -psicose 3-epimerase (DPEase) to convert D -fructose into D -allulose. Additionally, the process can also utilise D -tagatose 3-epimerase (DTEase). However, the process is not efficient due to the poor thermotolerance of the enzymes and low conversion rates between the sugars. This review describes three newly identified DAEases that possess desirable properties for the industrial-scale manufacturing of D -allulose. Other methods used to enhance process efficiency include the engineering of DAEases for improved thermotolerance or acid resistance, the utilization of Bacillus subtilis for the biosynthesis of D -allulose, and the immobilization of DAEases to enhance its activity, half-life, and stability. All these research advancements improve the yield of D -allulose, hence closing the gap between the small-scale production and industrial-scale manufacturing of D -allulose. |
