Blood Clotting Dissolution in the Presence of a Magnetic Field and Preliminary Study with MG63 Osteoblast-like Cells-Further Developments for Guided Bone Regeneration?

Autor: Di Gioia S; Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy., Milillo L; Independent Researcher, 70126 Bari, Italy., Hossain MN; Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy., Carbone A; Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy., Petruzzi M; Department of Interdisciplinary Medicine, University of Bari 'Aldo Moro', 70126 Bari, Italy., Conese M; Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy.
Jazyk: angličtina
Zdroj: Bioengineering (Basel, Switzerland) [Bioengineering (Basel)] 2023 Jul 26; Vol. 10 (8). Date of Electronic Publication: 2023 Jul 26.
DOI: 10.3390/bioengineering10080888
Abstrakt: Background: The influence of a magnetic field on the activation of bone cells and remodelling of alveolar bone is known to incite bone regeneration. Guided Bone Regeneration (GBR) aims to develop biomimetic scaffolds to allow for the functioning of the barrier and the precise succession of wound healing steps, including haemostasis. The effect of a magnetic field on blood clot dissolution has not been studied yet.
Methods: We conducted a methodological study on the clot stability in the presence of a static magnetic field (SMF). Preformed whole blood (WB) clots were treated with either a broad proteolytic enzyme (trypsin) or a specific fibrinolytic agent, i.e., tissue-type plasminogen activator (t-PA). MG63 osteoblast-like cells were added to preformed WB clots to assess cell proliferation.
Results: After having experienced a number of clotting and dissolution protocols, we obtained clot stability exerted by SMF when tissue factor (for clotting) and t-PA + plasminogen (for fibrinolysis) were used. WB clots allowed osteoblast-like cells to survive and proliferate, however no obvious effects of the magnetic field were noted.
Conclusions: Paramagnetic properties of erythrocytes may have influenced the reduction in clot dissolution. Future studies are warranted to fully exploit the combination of magnetic forces, WB clot and cells in GBR applied to orthodontics and prosthodontics.
Databáze: MEDLINE
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