Copper(II) Complexes with Carnosine Conjugates of Hyaluronic Acids at Different Dipeptide Loading Percentages Behave as Multiple SOD Mimics and Stimulate Nrf2 Translocation and Antioxidant Response in In Vitro Inflammatory Model.

Autor: Bellia F; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy., Lanza V; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy., Naletova I; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy., Tomasello B; Department of Drug and Health Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy., Ciaffaglione V; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy., Greco V; Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy., Sciuto S; Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy., Amico P; Fidia Farmaceutici SpA, Contrada Pizzuta, 96017 Noto, Italy., Inturri R; Fidia Farmaceutici SpA, Contrada Pizzuta, 96017 Noto, Italy., Vaccaro S; Fidia Farmaceutici SpA, Contrada Pizzuta, 96017 Noto, Italy., Campagna T; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy., Attanasio F; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy., Tabbì G; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy., Rizzarelli E; Institute of Crystallography, National Council of Research (CNR), P. Gaifami 18, 95126 Catania, Italy.; Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.
Jazyk: angličtina
Zdroj: Antioxidants (Basel, Switzerland) [Antioxidants (Basel)] 2023 Aug 18; Vol. 12 (8). Date of Electronic Publication: 2023 Aug 18.
DOI: 10.3390/antiox12081632
Abstrakt: A series of copper(II) complexes with the formula [Cu 2+ Hy( x )Car%] varying the molecular weight (MW) of Hyaluronic acid (Hy, x = 200 or 700 kDa) conjugated with carnosine (Car) present at different loading were synthesized and characterized via different spectroscopic techniques. The metal complexes behaved as Cu, Zn-superoxide dismutase (SOD1) mimics and showed some of the most efficient reaction rate values produced using a synthetic and water-soluble copper(II)-based SOD mimic reported to date. The increase in the percentage of Car moieties parallels the enhancement of the I 50 value determined via the indirect method of Fridovich. The presence of the non-functionalized Hy OH groups favors the scavenger activity of the copper(II) complexes with HyCar, recalling similar behavior previously found for the copper(II) complexes with Car conjugated using β-cyclodextrin or trehalose. In keeping with the new abilities of SOD1 to activate protective agents against oxidative stress in rheumatoid arthritis and osteoarthritis diseases, Cu 2+ interaction with HyCar promotes the nuclear translocation of erythroid 2-related factor that regulates the expressions of target genes, including Heme-Oxigenase-1, thus stimulating an antioxidant response in osteoblasts subjected to an inflammatory/oxidative insult.
Databáze: MEDLINE
Externí odkaz: