| Autor: |
Atang AE; Department of Chemistry, Oakland University, Rochester, MI 48309, USA., Keller AR; Department of Chemistry, Oakland University, Rochester, MI 48309, USA., Denha SA; Department of Chemistry, Oakland University, Rochester, MI 48309, USA., Avery AW; Department of Chemistry, Oakland University, Rochester, MI 48309, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Cells [Cells] 2023 Aug 19; Vol. 12 (16). Date of Electronic Publication: 2023 Aug 19. |
| DOI: |
10.3390/cells12162100 |
| Abstrakt: |
Spinocerebellar ataxia type 5 (SCA5) is a neurodegenerative disease caused by mutations in the SPTBN2 gene encoding the cytoskeletal protein β-III-spectrin. Previously, we demonstrated that a L253P missense mutation, localizing to the β-III-spectrin actin-binding domain (ABD), causes increased actin-binding affinity. Here we investigate the molecular consequences of nine additional ABD-localized, SCA5 missense mutations: V58M, K61E, T62I, K65E, F160C, D255G, T271I, Y272H, and H278R. We show that all of the mutations, similar to L253P, are positioned at or near the interface of the two calponin homology subdomains (CH1 and CH2) comprising the ABD. Using biochemical and biophysical approaches, we demonstrate that the mutant ABD proteins can attain a well-folded state. However, thermal denaturation studies show that all nine mutations are destabilizing, suggesting a structural disruption at the CH1-CH2 interface. Importantly, all nine mutations cause increased actin binding. The mutant actin-binding affinities vary greatly, and none of the nine mutations increase actin-binding affinity as much as L253P. ABD mutations causing high-affinity actin binding, with the notable exception of L253P, appear to be associated with an early age of symptom onset. Altogether, the data indicate that increased actin-binding affinity is a shared molecular consequence of numerous SCA5 mutations, which has important therapeutic implications. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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