The Efficacy of Transplanting Human Umbilical Cord Mesenchymal Stem Cell Sheets in the Treatment of Myocardial Infarction in Mice.

Autor:	Bui TQ; Cho Ray Hospital, Ho Chi Minh City 700000, Vietnam., Binh NT; Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Pham TL; Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.; Faculty of Biotechnology, Ho Chi Minh City Open University, Ho Chi Minh City 700000, Vietnam., Le Van T; Laboratory of Stem Cell Research and Application, University of Science, Ho Chi Minh City 700000, Vietnam.; Vietnam National University, Ho Chi Minh City 700000, Vietnam., Truong NH; Laboratory of Stem Cell Research and Application, University of Science, Ho Chi Minh City 700000, Vietnam.; Vietnam National University, Ho Chi Minh City 700000, Vietnam., Nguyen DP; Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Luu TT; Histology-Embryology-Pathology Department, Faculty of Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Nguyen-Xuan Pham T; Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam., Cam Tran T; Institute of Tropical Biology, Ho Chi Minh City 700000, Vietnam., Nguyen HT; Divison of Human and Animal Physiology, HCMC University of Education, Ho Chi Minh City 700000, Vietnam., Thuy-Trinh N; Vietnam National University, Ho Chi Minh City 700000, Vietnam.; School of Biomedical Engineering, International University, Ho Chi Minh City 700000, Vietnam., Tran PA; Interface Science and Materials Engineering Group, School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, Brisbane City, QLD 4000, Australia.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2023 Aug 03; Vol. 11 (8). Date of Electronic Publication: 2023 Aug 03.
DOI:	10.3390/biomedicines11082187
Abstrakt:	The transplantation of mesenchymal stem cell (MSC) sheets derived from human umbilical cords (hUCs) was investigated in this study as a potential application in treating myocardial infarction (MI). Two groups of hUC-MSC sheets were formed by populating LunaGel TM , which are 3D scaffolds of photo-crosslinkable gelatin-based hydrogel with two different cell densities. An MI model was created by ligating the left anterior descending coronary artery of healthy BALB/c mice. After two weeks, the cell sheets were applied directly to the MI area and the efficacy of the treatment was evaluated over the next two weeks by monitoring the mice's weight, evaluating the left ventricle ejection fraction, and assessing the histology of the heart tissue at the end of the experiment. Higher cell density showed significantly greater efficiency in MI mice treatment in terms of weight gain and the recovery of ejection fraction. The heart tissue of the groups receiving cell sheets showed human-CD44-positive staining and reduced fibrosis and apoptosis. In conclusion, the hUC-MSC sheets ameliorated heart MI injury in mice and the efficacy of the cell sheets improved as the number of cells increased.
Databáze:	MEDLINE
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