| Autor: |
Amrane-Abider M; Centre de Recherche en Technologies Agroalimentaires, Route de Targa Ouzemmour, Campus Universitaire, Bejaia 06000, Algeria., Imre M; Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences 'King Mihai I' from Timisoara, 300645 Timisoara, Romania., Herman V; Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences 'King Mihai I' from Timisoara, 300645 Timisoara, Romania., Debbou-Iouknane N; Department of Environment Biological Sciences, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria., Zemouri-Alioui S; Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria., Khaled S; Laboratory of Biomathematics, Biochemistry, Biophysics and Scientometrics, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria., Bouiche C; Centre de Recherche en Technologies Agroalimentaires, Route de Targa Ouzemmour, Campus Universitaire, Bejaia 06000, Algeria., Nerín C; Aragón Institute for Engineering Research (I3A), University of Zaragoza, Campus Rio Ebro, María de Luna 3, 50018 Zaragoza, Spain., Acaroz U; ACR Bio Food and Biochemistry Research and Development, Afyonkarahisar 03200, Turkey.; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey.; Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Kyrgyz-Turkish Manas University, Bishkek KG-720038, Kyrgyzstan., Ayad A; Department of Environment Biological Sciences, Faculty of Nature and Life Sciences, University of Bejaia, Bejaia 06000, Algeria. |
| Abstrakt: |
The objective of the present study is to identify the biochemical compounds extracted from OFI flowers using ultra-high-performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry and to evaluate their in vitro antioxidant activities and anticoccidial effects on the destruction of Eimeria oocysts isolated from naturally infected chickens. A domestic microwave was used with a refrigerant to condense the vapors generated during the extraction. The flavonoid and phenolic compound contents of the OFI flowers were determined according to standard methods. DPPH radical and H 2 O 2 scavenging capacities were used to assess the antioxidant activity. Regarding the anticoccidial activity, the Eimeria spp. oocysts used were isolated from the fresh feces of infected broilers and were determined in triplicate by incubation at an ambient temperature for 24 h. The results highlighted the considerable influence of the optimized acetone concentration, ratio, irradiation time, and microwave power parameters on the phenolic content and antioxidant activities. Our results revealed significant matches between the predicted and experimental values of the models. Molecular analysis revealed the presence of several biophenol classes such as quercetin, isorhamnetin 3-O-rutinoside, and quercetin-3-O-rutinoside. OFI flower extracts inhibited sporulation and damaged the morphology of Eimeria oocysts compared with normal sporulated Eimeria oocysts containing sporocysts. In conclusion, the optimized conditions were validated and found to fit very well with the experimental values. These findings suggest that the flowers of OFI should be considered sources of antioxidants. The results of the present study revealed that OFI flower extracts have anticoccidial activities against Eimeria -spp.-induced infection in broiler chickens. |
