Preanalytical variables influencing the interpretation and reporting of biological tests on blood samples of living and deceased donors for human body materials.

Autor: Padalko E; Department of Medical Microbiology, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium. elizaveta.padalko@uzgent.be.; Department of Diagnostic Sciences, Ghent University, C. Heymanslaan 10, 9000, Ghent, Belgium. elizaveta.padalko@uzgent.be.; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium. elizaveta.padalko@uzgent.be., Colenbie L; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Department of Transplant Center, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium., Delforge A; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Laboratory of Clinical Cellular Therapy, Institute J. Bordet, Rue Meylemeersch 90, 1070, Brussels, Belgium., Ectors N; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Faculty of Medicine, KU Leuven (Catholic University of Leuven), Oude Markt 13, 3000, Leuven, Belgium., Guns J; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Department of Laboratory Quality, Free University of Brussels VUB/University Hospital, Laarbeeklaan 101, 1090, Brussels, Belgium., Imbert R; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Department of Medically Assisted Reproduction, CHIREC, Boulevard du Triomphe 201, 1160, Brussels, Belgium., Jansens H; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Department of Medical Microbiology, Antwerp University/University Hospital, Drie Eikenstraat 655, 2650, Edegem, Belgium., Pirnay JP; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1, B-1120, Brussels, Belgium., Rodenbach MP; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Service du Sang, Croix-Rouge de Belgique, Rue du Fond du Maréchal 8, 5021, Suarlée, Belgium., Van Riet I; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Department of Hematology, University Hospital Brussels (UZ Brussel), Jette, Belgium., Vansteenbrugge A; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Department of Medically Assisted Reproduction, CHIREC, Boulevard du Triomphe 201, 1160, Brussels, Belgium., Verbeken G; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1, B-1120, Brussels, Belgium., Baltes M; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium., Beele H; Working Group on Cells, Tissues and Organs of the Superior Health Council, Brussels, Belgium.; Department of Dermatology, Ghent University Hospital, C. Heymanslaan 10, 9000, Ghent, Belgium.
Jazyk: angličtina
Zdroj: Cell and tissue banking [Cell Tissue Bank] 2024 Jun; Vol. 25 (2), pp. 509-520. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1007/s10561-023-10106-z
Abstrakt: With the present paper, the Working Group on Cells, Tissues and Organs and other experts of the Superior Health Council of Belgium aimed to provide stakeholders in material of human origin with advice on critical aspects of serological and nucleic acid test (NAT) testing, to improve virological safety of cell- and tissue and organ donation. The current paper focusses on a number of preanalytical variables which can be critical for any medical biology examination: (1) sampling related variables (type of samples, collection of the samples, volume of the sample, choice of specific tubes, identification of tubes), (2) variables related to transport, storage and processing of blood samples (transport, centrifugation and haemolysis, storage before and after centrifugation, use of serum versus plasma), (3) variables related to dilution (haemodilution, pooling of samples), and (4) test dependent variables (available tests and validation). Depending on the type of donor (deceased donor (heart-beating or non-heart beating) versus living donor (allogeneic, related, autologous), and the type of donated human material (cells, tissue or organs) additional factors can play a role: pre- and post-mortem sampling, conditions of sampling (e.g. morgue), haemodilution, possibility of retesting.
(© 2023. The Author(s).)
Databáze: MEDLINE
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