The method for assessment of local permutations in the glomerular patterns of the rat olfactory bulb by aligning interindividual odor maps.

Autor: Matukhno AE; Research Center for Neurotechnology, Southern Federal University, Rostov-on-Don, 344090, Russia. aematuhno@sfedu.ru., Petrushan MV; Research Center for Neurotechnology, Southern Federal University, Rostov-on-Don, 344090, Russia., Kiroy VN; Research Center for Neurotechnology, Southern Federal University, Rostov-on-Don, 344090, Russia., Arsenyev FV; Advanced Research Foundation, Moscow, 121059, Russia., Lysenko LV; Research Center for Neurotechnology, Southern Federal University, Rostov-on-Don, 344090, Russia.; Department of Physics, Southern Federal University, Rostov-on-Don, 344090, Russia.
Jazyk: angličtina
Zdroj: Journal of computational neuroscience [J Comput Neurosci] 2023 Nov; Vol. 51 (4), pp. 433-444. Date of Electronic Publication: 2023 Aug 25.
DOI: 10.1007/s10827-023-00858-8
Abstrakt: The comparison of odor functional maps in rodents demonstrates a high degree of inter-individual variability in glomerular activity patterns. There are substantial methodological difficulties in the interindividual assessment of local permutations in the glomerular patterns, since the position of anatomical extracranial landmarks, as well as the size, shape and angular orientation of olfactory bulbs can vary significantly. A new method for defining anatomical coordinates of active glomeruli in the rat olfactory bulb has been developed. The method compares the interindividual odor functional maps and calculates probabilistic maps of glomerular activity with adjustment. This adjustment involves rotation, scaling and shift of the functional map relative to its expected position in probabilistic map, computed according to the anatomical coordinates. The calculation of the probabilistic map of the odorant-specific response compensates for potential anatoamical errors due to individual variability in olfactory bulb dimensions and angular orientation. We show its efficiency on real data from a large animal sample recorded by two-photon calcium imaging in dorsal surface of the rat olfactory bulb. The proposed method with probabilistic map calculation enables the spatial consistency of the effects of individual odorants in different rats to be assessed and allow stereotypical positions of odor-specific clusters in the glomerular layer of the olfactory bulb to be identified.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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