| Autor: |
Yao W; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.; Research Institute for Food Nutrition and Human Health (111 Center), Guangzhou 510640, China., Yong J; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.; Research Institute for Food Nutrition and Human Health (111 Center), Guangzhou 510640, China., Lv B; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.; Research Institute for Food Nutrition and Human Health (111 Center), Guangzhou 510640, China., Guo S; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.; Research Institute for Food Nutrition and Human Health (111 Center), Guangzhou 510640, China., You L; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.; Research Institute for Food Nutrition and Human Health (111 Center), Guangzhou 510640, China., Cheung PC; Food & Nutritional Sciences Program, School of Life Sciences, Chinese University of Hong Kong, Hong Kong 999077, China., Kulikouskaya VI; Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, 36 Skaryna Str., 220141 Minsk, Belarus. |
| Abstrakt: |
The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H 2 O 2 ) treatment was used to prepare low-molecular-weight seaweed polysaccharides from Sargassum fusiforme . The effects of UV/H 2 O 2 treatment on the physicochemical properties and anti-photoaging activity of S. fusiforme polysaccharides were studied. UV/H 2 O 2 treatment effectively degraded polysaccharides from S. fusiforme (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of S. fusiforme polysaccharides were investigated using human epidermal HaCaT cells in vitro . DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H 2 O 2 treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H 2 O 2 treatment could effectively improve the anti-photoaging activity of S. fusiforme polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides. |
