| Autor: |
Gurgel RS; Programa Graduate Program in Biodiversity and Biotechnology of the Bionorte Network, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil.; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil., de Melo Pereira DÍ; Programa Graduate Program in Biodiversity and Biotechnology of the Bionorte Network, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil.; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil., Garcia AVF; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil., Fernandes de Souza AT; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil.; Graduate Program in Biotechnology and Natural Resources of the Amazon, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil., Mendes da Silva T; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil., de Andrade CP; Programa Graduate Program in Biodiversity and Biotechnology of the Bionorte Network, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil.; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil., Lima da Silva W; Bioprospection and Biotechnology Laboratory, National Institute of Amazonian Research, Manaus 69067-375, Brazil., Nunez CV; Graduate Program in Biotechnology and Natural Resources of the Amazon, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil.; Bioprospection and Biotechnology Laboratory, National Institute of Amazonian Research, Manaus 69067-375, Brazil., Fantin C; Graduate Program in Biotechnology and Natural Resources of the Amazon, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil.; Multicentric Graduate Program in Biochemistry and Molecular Biology, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil., de Lima Procópio RE; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil.; Graduate Program in Biotechnology and Natural Resources of the Amazon, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil., Albuquerque PM; Programa Graduate Program in Biodiversity and Biotechnology of the Bionorte Network, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil.; Research Group on Chemistry Applied to Technology, School of Technology, Amazonas State University, Manaus 69050-020, Brazil.; Graduate Program in Biotechnology and Natural Resources of the Amazon, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil.; Multicentric Graduate Program in Biochemistry and Molecular Biology, School of Health Sciences, Amazonas State University, Manaus 69050-010, Brazil. |
| Abstrakt: |
The endophytic fungal community of the Amazonian medicinal plant Arrabidaea chica (Bignoniaceae) was evaluated based on the hypothesis that microbial communities associated with plant species in the Amazon region may produce metabolites with interesting bioactive properties. Therefore, the antimicrobial and antioxidant activities of the fungal extracts were investigated. A total of 107 endophytic fungi were grown in liquid medium and the metabolites were extracted with ethyl acetate. In the screening of fungal extracts for antimicrobial activity, the fungus identified as Botryosphaeria mamane CF2-13 was the most promising, with activity against E. coli , S. epidermidis , P. mirabilis , B. subtilis , S. marcescens , K. pneumoniae , S. enterica , A. brasiliensis , C. albicans , C. tropicalis and, especially, against S. aureus and C. parapsilosis (MIC = 0.312 mg/mL). Screening for antioxidant potential using the DPPH elimination assay showed that the Colletotrichum sp. CG1-7 endophyte extract exhibited potential activity with an EC 50 of 11 µg/mL, which is equivalent to quercetin (8 µg/mL). The FRAP method confirmed the antioxidant potential of the fungal extracts. The presence of phenolic compounds and flavonoids in the active extracts was confirmed using TLC. These results indicate that two of the fungi isolated from A. chica exhibit significant antimicrobial and antioxidant potential. |
