| Autor: |
Iturritxa E; Department of Forest Science, Neiker-BRTA, Instituto Vasco de Investigación y Desarrollo Agrario, Granja Modelo s/n, Antigua Carretera Nacional 1, Km. 355, 01192 Arkaute, Spain.; Grup Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, c/Marcel⋅lí Domingo 1, 43007 Tarragona, Spain., Mesanza N; Department of Forest Science, Neiker-BRTA, Instituto Vasco de Investigación y Desarrollo Agrario, Granja Modelo s/n, Antigua Carretera Nacional 1, Km. 355, 01192 Arkaute, Spain., Torija MJ; Grup Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, c/Marcel⋅lí Domingo 1, 43007 Tarragona, Spain. |
| Abstrakt: |
Native wild yeasts from forest ecosystems represent an interesting potential source of biocontrol organisms in synergy with disease-tolerant forest materials. Yeasts have a combination of competitive mechanisms and low requirements for their biotechnological application as biocontrol agents. The current study aimed to increase the number of biocontrol candidates against Fusarium circinatum and Diplodia sapinea . The enzymatic and antagonistic activities of the biocontrol candidates were evaluated using different screening methods, in which the direct impact on the growth of the pathogen was measured as well as some properties such as cellulose and lignin degradation, tolerance to biocides, volatile compound production, or iron effect, which may be of interest in biotechnological processes related to the management of forest diseases. A total of 58 yeast strains belonging to 21 different species were obtained from oak forest and vineyard ecosystems and evaluated. The application of yeast treatment behaved differently depending on the pathogen and the plant clone. The 2g isolate ( Torulaspora delbrueckii ) showed the highest inhibitory activity for D. sapinea and 25q and 90q ( Saccharomyces paradoxus ) for F. circinatum . Clones IN416 and IN216 were the most susceptible and the most tolerant to D. sapinea , respectively, while the opposite was observed for F. circinatum . |
